APC/Cyanine7 Rabbit anti-Human CD10 Monoclonal Antibody for FC - P08473

Core Product Specifications and Parameters

Biological Background: CD10 Function and Localization

• Neprilysin (CD10) is a membrane-bound zinc metalloprotease that preferentially cleaves peptide bonds on the amino side of hydrophobic residues, with a preference for substrates shorter than 30 amino acids. Related references: PMID:15283675, PMID:6208535, PMID:6349683
• It plays a critical role in the inactivation of opioid peptides such as Met- and Leu-enkephalins by cleaving the Gly-Phe bond, thereby regulating pain and neuronal signaling. Related references: PMID:17101991, PMID:6349683
• Substrates also include bradykinin, substance P, neurotensin, angiotensins I/II/(1-9), atrial natriuretic factor (ANF), and brain natriuretic peptide (BNP), implicating it in cardiovascular and blood pressure control. Related references: PMID:6208535, PMID:15283675, PMID:6349683, PMID:16254193, PMID:2531377, PMID:2972276
• Located at the cell membrane, its extracellular domain catalyzes peptide hormone degradation at the cell surface.
• Post-translational modifications include glycosylation, phosphorylation, myristoylation, and disulfide bonds, which may influence its stability and activity.
• UV irradiation induces elastase activity in neprilysin, contributing to the degradation of skin preelastic and elastic fibers. Related references: PMID:20876573
• Mutations in MME are associated with Charcot-Marie-Tooth disease type 2T and spinocerebellar ataxia type 43, highlighting roles beyond peptidase activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the large extracellular region (amino acids 52–750) of human CD10, suggesting that the antibody likely recognizes an epitope accessible on live cells, making it suitable for flow cytometry.
• For flow cytometry, consider using human cell lines known to express high levels of CD10, such as B-cell leukemia lines or renal epithelial cells. Validate expression in your sample system prior to experimental use.
• As an APC/Cyanine7 conjugate, careful compensation and minimal light exposure are recommended. Titrate the antibody to determine the optimal working dilution for your specific cell model and cytometer setup.
• Isotype controls and Fc receptor blocking may reduce non-specific binding in certain sample types; include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop IVD raw-material sourcing solution, covering everything from antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and rare target sourcing. This anti-CD10 antibody supports research and diagnostic development in cardiovascular, neurodegenerative, and oncology areas where neprilysin signaling is critical. As your trusted partner from concept to clinic, CamelBio ensures streamlined access to high-quality reagents tailored to your assay needs.