APC/Cyanine7 Anti-Mouse CD69 Monoclonal Antibody for Flow Cytometry - P37217

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• Early activation antigen CD69 is a transmembrane receptor rapidly expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, platelets, and eosinophils. Related references: PMID:38540778
• It plays a central role in immune homeostasis by modulating T-cell differentiation, including negative regulation of Th17 cell development through interaction with galectin-1 on dendritic cells, which involves the JAK3/STAT5 signaling pathway and RORC transcription. Related references: PMID:20696842
• CD69 promotes regulatory T-cell conversion via the S100A8/S100A9 complex on peripheral blood mononuclear cells and acts as an oxidized low-density lipoprotein (oxLDL) receptor to induce PDCD1 expression.
• In eosinophils, it induces IL-10 production through the ERK1/2 pathway, contributing to anti-inflammatory responses. Related references: PMID:38540778
• It negatively regulates chemotactic responses by acting as a sphingosine 1-phosphate receptor 1 (S1PR1) agonist, facilitating receptor internalization and degradation. Related references: PMID:16525420
• Participates in adipose tissue-derived mesenchymal stem cell‑mediated protection against Pseudomonas aeruginosa infection by pathogen recognition and ERK1‑dependent granulocyte‑macrophage colony‑stimulating factor secretion.
• Localizes to the cell membrane as a single‑pass type II transmembrane protein and undergoes post‑translational modifications including glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 64–199 of mouse CD69, covering part of the extracellular domain; this design may favor recognition of the native conformation on live cells during flow cytometry.
• For flow cytometry applications, ensure proper compensation controls are used for the APC/Cyanine7 tandem dye and that cells remain viable throughout staining and acquisition.
• CD69 is rapidly upregulated upon cellular activation; careful sample handling and timely processing are recommended to avoid unintended ex vivo activation and ensure accurate detection.
• Cross-reactivity has been confirmed for mouse; for other species, validation in the relevant biological system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one‑stop supply platform for IVD raw materials, including high‑quality antibodies like this APC/Cyanine7‑conjugated anti‑mouse CD69 monoclonal antibody, optimized for flow cytometry‑based immune phenotyping. In addition to individual antibodies, we offer validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to support your diagnostic and research workflows. Partner with CamelBio for dependable access to critical raw materials from concept to clinic.