APC/Cyanine7 Anti-Mouse CD45 Rabbit Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• Receptor-type tyrosine-protein phosphatase C (CD45), encoded by the PTPRC gene, is a transmembrane phosphatase essential for signal transduction in immune cells. Alternative splicing generates multiple isoforms (e.g., CD45R, B220) with distinct extracellular domains.
• CD45 is required for T-cell activation through the antigen receptor, acting as a positive regulator upon binding to DPP4. It modulates the threshold of T-cell receptor signaling.
• The intracellular region contains two protein tyrosine phosphatase (PTP) domains; the first possesses catalytic activity, while the second influences substrate specificity and activity.
• CD45 dephosphorylates Src family kinases such as LYN, FYN, and LCK, thereby regulating their kinase activities and downstream signaling cascades in lymphocytes.
• Interaction with CLEC10A on antigen-presenting cells (e.g., immature dendritic cells) fine-tunes T-cell activation, contributing to the maintenance of self-tolerance.
• CD45 is localized to the cell membrane and enriched in membrane rafts and the immunological synapse, positioning it for receptor signal integration.
• Post-translational modifications include extensive N- and O-linked glycosylation, which influences isoform expression and protein-protein interactions.
• Known as leukocyte common antigen (L-CA) and Ly-5, CD45 is broadly expressed on all nucleated hematopoietic cells, with isoform-specific patterns distinguishing leukocyte subsets.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 26–566 of mouse CD45, covering a large portion of the extracellular domain. This design likely enables recognition of multiple CD45 isoforms, but users should verify isoform specificity in their system.
• For flow cytometry, titrate the APC/Cyanine7-conjugated antibody on mouse leukocytes to determine the optimal staining concentration, and include appropriate isotype and fluorescence-minus-one (FMO) controls.
• APC/Cyanine7 emits in the far-red channel (~780 nm), allowing clear separation from common fluorophores like FITC, PE, and APC. Use a compatible laser line (e.g., 633 nm excitation) and appropriate filter sets.
• Due to high CD45 expression on leukocytes, avoid over-staining which can cause non-specific binding; a typical starting dilution of 0.25–1.0 µg per million cells in flow cytometry is a reasonable reference point.
• For panel design, consider CD45 as a pan-leukocyte marker to gate live immune cells, then combine with lineage-specific antibodies (e.g., CD3, CD19) for immunophenotyping.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD45 monoclonal antibody supports immunological research, diagnostic assay development, and immune cell profiling. Beyond this product, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, empowering your projects with reliable and scalable supply solutions.