APC/Cyanine7 Anti-Mouse CD38 Monoclonal Antibody for Flow Cytometry - P56528

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 is a multifunctional transmembrane glycoprotein also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, I-19, ADPRC 1, and NIM-R5 antigen.
• It enzymatically synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion, and nicotinate-adenine dinucleotide phosphate (NAADP+), a Ca2+ mobilizer. Related references: PMID:11829748
• CD38 also exhibits cADPR hydrolase activity, cleaving cADPR to ADP-ribose, and functions as a receptor binding CD31 on endothelial cells to promote lymphocyte activation and migration.
• It belongs to the ADP-ribosyl cyclase family and is encoded by the Cd38 gene in mouse.
• Subcellularly, it localizes to the membrane as a single-pass type II transmembrane protein with a signal-anchor sequence.
• Key post-translational modifications include disulfide bonds and N-linked glycosylation, contributing to its structural stability and function.
• In dendritic cells, CD38 regulates CCL21-driven migration, survival, and Th1 polarization, while in lamina propria T cells, it triggers LCK/LAT signaling via CD31 interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein; consider using the full-length or a specific extracellular domain for immunization design.
• This antibody is suitable for flow cytometry (FC); validate optimal dilution and staining conditions in the relevant mouse sample system.
• For functional studies, ensure cell surface retention of CD38, and avoid methanol permeabilization unless specifically required.
• Combine with other lineage markers for detailed immunophenotyping of lymphocytes or dendritic cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies for CD38 and other immune checkpoint targets, supporting diagnostic developers and research labs. As a dedicated B2B partner, CamelBio ensures reliable supply of bulk ancillary reagents and provides rare-target raw-material sourcing to accelerate your projects from concept to clinic. Our expertise in immunology targets like CD38 helps streamline your assay development and scale-up.