APC/Cyanine7 Anti-Human/Monkey CD127/IL-7Rα Monoclonal Antibody for Flow Cytometry - P16871

Core Product Specifications and Parameters

Biological Background: CD127/IL-7Rα Function and Localization

• IL7R encodes the interleukin-7 receptor subunit alpha (CD127), a type I cytokine receptor that mediates signaling for interleukin-7 (IL-7) and thymic stromal lymphopoietin (TSLP).
• The receptor is critical for lymphocyte development, survival, and homeostasis, playing essential roles in T-cell and B-cell biology.
• Alternative splicing generates multiple isoforms, including membrane-bound and secreted forms (sIL-7R), which may modulate IL-7 bioavailability.
• CD127 is predominantly expressed on the cell surface, consistent with its function as a transmembrane receptor; the secreted isoform lacks the transmembrane domain.
• Post-translational modifications include glycosylation and phosphorylation, which can influence receptor stability, ligand binding, and signal transduction.
• Disease-associated variants in IL7R are linked to severe combined immunodeficiency (SCID), highlighting its non-redundant role in immune function.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry (FC), with reported cross-reactivity to human and cynomolgus monkey CD127.
• The immunogen corresponds to the extracellular domain (amino acids 21–236), suggesting recognition of native surface-expressed receptor.
• For FC, we recommend titrating the antibody to determine the optimal working concentration and including appropriate isotype controls.
• Consider using freshly isolated cells or well-characterized cell lines to ensure robust staining; fixable viability dyes may improve discrimination of live/dead cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies and ancillary reagents to support immunology and diagnostic development. This APC/Cyanine7 anti-CD127 monoclonal antibody empowers flow cytometry-based research into lymphocyte biology and immune disorders. Contact us to explore bulk supply options or custom conjugate requests tailored to your workflow.