APC/Cyanine7 Anti-Human CD38 Rabbit Monoclonal Antibody for Flow Cytometry - P28907

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1), also known as ADPRC1, cADPR1, and T10, is a ~34 kDa (also detected at ~13 kDa) transmembrane glycoprotein encoded by the CD38 gene (GeneID: 952).
• It functions as an ectoenzyme with ADP-ribosyl cyclase activity, synthesizing the second messenger cyclic ADP-ribose (cADPR) from NAD+, which plays a critical role in glucose-induced insulin secretion. Related references: PMID:7961800, PMID:8253715
• CD38 also catalyzes the production of the Ca2+-mobilizing messenger nicotinate-adenine dinucleotide phosphate (NAADP+) from NADP+ and nicotinic acid, with a preference for 2'-phospho-cADPR as substrate. Related references: PMID:16690024
• In addition, CD38 exhibits cADPR hydrolase activity, contributing to the regulation of intracellular cADPR levels. Related references: PMID:7961800, PMID:8253715
• Beyond enzymatic functions, CD38 acts as a receptor for CD31 (PECAM-1) on endothelial cells, facilitating lymphocyte adhesion, activation, and transendothelial migration. Related references: PMID:9551996
• In dendritic cells, CD38 signaling modulates mature-stage functions such as CCL21-driven migration, survival, and Th1-polarizing activity. Related references: PMID:16293598
• CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, and certain malignancies including lymphoma and neuroblastoma.
• Subcellularly, CD38 is a cell surface and membrane-localized type II transmembrane protein.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 43-300), which is expected to be accessible on the surface of live cells, making this antibody suitable for flow cytometry without permeabilization.
• When using the APC/Cyanine7 conjugate, protect the antibody from light and avoid freeze-thaw cycles to maintain tandem dye stability and minimize signal loss.
• For optimal flow cytometry results, titrate the antibody to determine the best concentration for your specific cell type and staining conditions, and include appropriate single-stained compensation controls to account for spectral overlap of APC/Cyanine7.
• Validate reactivity in known CD38-expressing human cell lines (e.g., Jurkat, RPMI-8226, or activated T cells) or primary samples, and confirm specificity using CD38-negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials. Our CD38-targeting antibodies, including conjugated formats, support applications in immunology and oncology research, with reliable supply for assay development. From validated monoclonal antibodies to bulk ancillary reagents, we help you bridge the gap between concept and clinic.