APC/Cyanine7 Anti-HLA-DRA Monoclonal Antibody for Flow Cytometry - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DRA Function and Localization

• The HLA-DRA gene encodes the alpha chain of MHC class II molecule HLA-DR, which pairs with the beta chain (HLA-DRB) to form functional heterodimers on antigen-presenting cells. This complex presents peptide antigens to CD4+ T cells, orchestrating T-helper effector functions, antibody responses, and macrophage activation (PubMed:15265931, PubMed:15322540, PubMed:17334368). Related references: PMID:15265931 PMID:15322540 PMID:17334368
• Typically presents extracellular peptides of 10–30 amino acids derived from endocytosed and lysosomally processed antigens, with a hydrophobic anchor residue at position 1 being critical for binding (PubMed:8145819). Related references: PMID:8145819
• In the tumor microenvironment, HLA-DR presents peptides from apoptotic tumor cells or secreted tumor proteins taken up by APCs, influencing anti-tumor immunity (PubMed:31495665). Related references: PMID:31495665
• Additionally, HLA-DR presents peptides from intracellular proteins via macroautophagy, a process essential for thymic T cell selection and maintenance of central immune tolerance (PubMed:17182262, PubMed:23783831). Related references: PMID:17182262 PMID:23783831
• Subcellular localization spans the cell membrane, endosomal/lysosomal membranes, and autolysosomes, reflecting its dynamic role in antigen processing and presentation.
• Expressed on professional antigen-presenting cells (macrophages, dendritic cells, B cells) and thymic epithelial cells (at protein level) (PubMed:15322540, PubMed:23783831, PubMed:31495665). Related references: PMID:15322540 PMID:23783831 PMID:31495665
• The protein features an N-terminal signal sequence, a single transmembrane helix, and two disulfide bonds in the extracellular domain; it undergoes N-linked glycosylation and can be ubiquitinated.

Experimental Guidance and Technical Tips

• This APC/Cyanine7-conjugated monoclonal antibody is raised against recombinant human HLA-DR; it is likely to recognize a conformational epitope. For flow cytometry, use standard surface staining protocols on live cells, keeping antibodies and cells cold to prevent antigen internalization.
• Given the conserved cross-reactivity with cynomolgus monkey, consider titration experiments for both human and NHP samples to determine optimal working concentration.
• To minimize non-specific binding in myeloid-rich samples, include an Fc receptor blocking step (e.g., human TruStain FcX or similar) and appropriate isotype controls.
• The 29 kDa band (HLA-DRA chain) is often detected as part of the MHCII α/β heterodimer on the cell surface; under reducing conditions, the alpha chain may run at a slightly higher apparent molecular weight due to glycosylation.
• For intracellular staining, mild fixation/permeabilization is required; validate the protocol in your sample system as the epitope may be sensitive to harsh treatments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and labs comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents for immunophenotyping and MHC research. This HLA-DRA product supports flow cytometry applications for human and non-human primate immune profiling. We also specialize in sourcing rare-target raw materials, enabling your workflow from concept to clinic with expert technical consultation.