APC/Cyanine7 Anti-CD45 Monoclonal Antibody for Flow Cytometry - P08575-8

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• Isoform 6 of receptor-type tyrosine-protein phosphatase C, commonly known as CD45, is a leukocyte-specific transmembrane glycoprotein also referred to as leukocyte common antigen (L-CA) and T200.
• CD45 is encoded by the PTPRC gene and acts as a receptor-linker phosphatase, playing a critical role in signal transduction by dephosphorylating phosphotyrosine residues.
• Alternative splicing yields multiple isoforms, including isoform 6 highlighted here, with a predicted molecular weight of 138 kDa.
• Widely expressed on all nucleated hematopoietic cells, CD45 is a fundamental marker for leukocyte populations in immunophenotyping and diagnostic applications.

Experimental Guidance and Technical Tips

• For flow cytometry applications, titrate this antibody in a dilution series (e.g., 0.1–5 µg/mL) to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Validate the antibody in your target species sample systems, considering the documented cross-reactivity with human and cynomolgus CD45.
• Use appropriate isotype controls and gating strategies to distinguish CD45-positive leukocyte populations from background signals in heterogeneous samples.
• Consider antigen integrity during sample preparation; CD45 epitopes may be sensitive to certain fixation or permeabilization protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials, offering validated monoclonal antibodies, antibody pairs, and ancillary reagents for diagnostic manufacturers and research laboratories. We specialize in bridging the gap from concept to clinic, with particular expertise in leukocyte marker development and immunophenotyping raw materials. For your CD45-based projects, we can support needs ranging from custom conjugation and bulk supply to technical consultation and rare target sourcing.