APC/Cyanine7 Anti-CD3E Rabbit Monoclonal Antibody for Intracellular Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3E Function and Localization

• Human CD3E encodes the CD3 epsilon chain, a core component of the TCR-CD3 complex on T-lymphocytes, essential for adaptive immune responses (PubMed:15294938, PubMed:15546002, PubMed:2470098).
  Related references: PMID:15294938, PMID:15546002, PMID:2470098
• Upon TCR engagement by antigen-presenting cells, CD3E cytoplasmic ITAM motifs are phosphorylated by Src family kinases (LCK/FYN), creating docking sites for ZAP70 and initiating downstream signaling cascades (PubMed:2470098, PubMed:40592325).
  Related references: PMID:2470098, PMID:40592325
• CD3E is critical for proper T-cell development, participating in both positive and negative selection in the thymus (By similarity).
• The protein also regulates TCR-CD3 surface expression by mediating receptor internalization and down-regulation via endocytosis motifs in its cytosolic region (PubMed:10384095, PubMed:26507128).
  Related references: PMID:10384095, PMID:26507128
• Beyond canonical TCR signaling, CD3E acts as a receptor for ITPRIPL1, which inhibits T-cell activation by recruiting NCK1 and blocking ZAP70 interaction and the ERK-NFkB pathway (PubMed:12110186, PubMed:38614099).
  Related references: PMID:12110186, PMID:38614099
• CD3E is a single-pass type I transmembrane glycoprotein located on the cell membrane; it contains an immunoglobulin-like extracellular domain, a transmembrane helix, and a cytoplasmic tail with ITAMs.
• The mature protein has an apparent molecular weight of ~23 kDa (UniProt calculated mass: 23,147 Da) and is subject to phosphorylation on ITAM tyrosine residues.

Experimental Guidance and Technical Tips

• This antibody is conjugated to APC/Cyanine7 and validated for intracellular flow cytometry (FC intra); fix and permeabilize cells according to your standard protocol prior to staining.
• The immunogen spans amino acids 50–150 within the extracellular domain of CD3E; verify specificity by comparing positive (e.g., human PBMCs) and negative (CD3E knockout) samples or by pre-incubating with blocking peptide.
• Titrate the antibody to determine the optimal staining concentration for your sample; use appropriate isotype controls and fluorescence compensation when building multicolor panels.
• Since CD3E surface expression is rapidly downregulated upon T-cell activation, confirm the cellular activation status and ensure adequate permeabilization to detect intracellular pools if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material supply, connecting diagnostic manufacturers and research labs with high-quality antibodies, reagents, and technical support. This anti-CD3E monoclonal antibody exemplifies our offering for T-cell immunophenotyping and adaptive immunity research. From validated antibody pairs and bulk recombinant proteins to custom sourcing of rare targets, we provide end-to-end support to move your assay from concept to clinic.