APC/Cyanine7 Anti-CD20 Monoclonal Antibody for Flow Cytometry - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• B-lymphocyte antigen CD20 (also known as MS4A1, B1, Bp35, Leu-16) is a member of the membrane-spanning 4A family, with a major isoform of approximately 33 kDa derived from alternative splicing.
• CD20 functions as a store-operated calcium channel component, regulating calcium influx essential for B-cell development, differentiation, and activation downstream of B-cell receptor signaling. Related references: PMID:12920111 PMID:3925015 PMID:7684739 PMID:18474602
• The protein is localized exclusively to the cell membrane, consistent with its role as a surface antigen.
• Post-translational modifications include palmitoylation, phosphorylation, and disulfide bond formation, which may influence membrane association and signaling.
• Expression of CD20 is restricted to B-lymphocytes, making it a specific marker for B-cell lineages.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an intracellular domain of CD20 (amino acids 100–200); consider permeabilization protocols if intracellular staining is required for flow cytometry.
• Titrate the antibody to determine the optimal concentration for your specific flow cytometry assay and sample type.
• The antibody cross-reacts with cynomolgus CD20; validate staining in the relevant sample matrix if using non-human primate models.
• Store and handle according to recommended cold-chain conditions; avoid repeated freeze-thaw cycles to maintain conjugate stability.

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CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for diagnostic development. With deep expertise in B-cell markers such as CD20, we support researchers and manufacturers in securing high-quality materials from concept to clinic. Whether you need rare-target antibodies or scalable supply solutions, CamelBio bridges your sourcing requirements at every stage.