APC/Cyanine7 Anti-CD185/CXCR5 Monoclonal Antibody for FC - P32302

Core Product Specifications and Parameters

Biological Background: CD185/CXCR5 Function and Localization

• CD185/CXCR5 is a C-X-C chemokine receptor type 5, also known as Burkitt lymphoma receptor 1 (BLR1) or monocyte-derived receptor 15 (MDR-15).
• It functions as a cytokine receptor that specifically binds B-lymphocyte chemoattractant (BLC), guiding B-cell migration into B-cell follicles of spleen and Peyer's patches.
• CXCR5 belongs to the G protein-coupled receptor (GPCR) family, characterized by seven transmembrane domains, and plays a role in B-cell activation and differentiation.
• The receptor is predominantly expressed on mature B-cells and Burkitt lymphoma cells, suggesting involvement in B-cell lymphomagenesis.
• Subcellular localization is primarily at the cell membrane, consistent with its role as a transmembrane receptor.
• According to UniProt, CXCR5 is subject to alternative splicing and contains disulfide bonds, which are common structural features of chemokine receptors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1-47), which may provide good specificity for the native receptor on live cells.
• As a directly conjugated APC/Cyanine7 antibody, it is suitable for multicolor flow cytometry panels; consider titrating to determine optimal concentration for your specific cell type.
• For best results, use fresh or properly cryopreserved human PBMCs or B-cell lines, and include Fc receptor blocking to reduce non-specific binding.
• Note that CXCR5 expression can be modulated upon B-cell activation; validate staining in your experimental system with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials, including validated antibodies for immune cell profiling. This APC/Cyanine7 anti-CXCR5 antibody supports flow cytometry-based applications in immunology and oncology research. As a comprehensive sourcing partner, we offer customized monoclonal antibodies, bulk reagents, and technical support to accelerate your assay development.