APC Rat anti-Mouse Ly6G Monoclonal Antibody for Flow Cytometry - P35461

Core Product Specifications and Parameters

Biological Background: Lymphocyte antigen 6G Function and Localization

• Lymphocyte antigen 6G (Ly-6G), also known as Ly-6G.1 or Gr-1, is encoded by the Ly6g gene.
• It belongs to the Ly-6 family of proteins, which share a conserved cysteine-rich domain stabilized by disulfide bonds.
• Ly-6G is a glycoprotein anchored to the outer leaflet of the plasma membrane via a glycosylphosphatidylinositol (GPI) moiety.
• The protein localizes to the cell membrane, consistent with its role as a surface antigen.
• Tissue expression is restricted to bone marrow, as indicated by its tissue specificity.
• The precursor protein contains a signal peptide for translocation into the endoplasmic reticulum, where GPI attachment and N‑glycosylation occur.
• The calculated molecular weight of the unprocessed polypeptide is approximately 14.3 kDa (14,324 Da).

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular region (amino acids 27‑105) of mouse Ly-6G, which includes the proposed ligand‑binding domain; thus, the antibody may recognize the native, surface‑exposed epitope.
• For flow cytometry applications, consider titrating the antibody to determine the optimal concentration for your specific experimental setup.
• Always include appropriate isotype controls, fluorescence compensation controls, and viability dyes to ensure accurate gating of Ly‑6G‑positive populations.
• Validate staining specificity in the relevant mouse tissue or cell line, as cross‑reactivity with other Ly‑6 family members (e.g., Ly‑6C) has been reported for some anti‑Gr‑1 clones.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers with comprehensive access to raw materials, including this APC‑conjugated anti‑mouse Ly6G monoclonal antibody suitable for flow cytometric immunophenotyping. As a single‑source partner, we offer validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare‑target raw materials, helping you streamline assay development from concept to clinic.