APC Rat anti-Mouse Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC - P35461 / P0CW02

Core Product Specifications and Parameters

Biological Background: Ly-6G Function and Localization

• Lymphocyte antigen 6G (Ly-6G), also known as Ly-6G.1, is a mouse protein encoded by the Ly6g gene.
• It is a GPI-anchored cell surface glycoprotein expressed on the plasma membrane, as indicated by its subcellular location and presence of a GPI-anchor.
• Tissue specificity data shows expression in bone marrow, consistent with its role in myeloid cells.
• As a member of the Ly-6/uPAR superfamily, Ly-6G contains conserved disulfide bond motifs and is glycosylated.
• The protein has a molecular weight of approximately 14.3 kDa (unprocessed precursor) and likely 15 kDa after post-translational modifications, with an 8 kDa counterpart possibly representing Ly-6C or a processed form.
• Ly-6G is commonly used as a granulocyte marker (Gr-1) and is often co-expressed with Ly-6C, which marks monocytes and subsets of granulocytes.
• No functional details are available from the reviewed UniProt entry, but the protein is annotated with keywords: Cell membrane, Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Membrane, Reference proteome, Signal.

Experimental Guidance and Technical Tips

• Since this is an APC-conjugated antibody, avoid repeated freeze-thaw cycles and protect from light during storage and use.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell population and experimental conditions.
• Validate staining in mouse bone marrow or peripheral blood samples, where Ly-6G/Ly-6C expression is well-characterized.
• Use appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• Consider co-staining with other myeloid markers (e.g., CD11b) for multi-parameter analysis.

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