APC Rat Anti-Mouse F4/80 Monoclonal Antibody for Flow Cytometry - Q61549

Core Product Specifications and Parameters

Biological Background: Adhesion G protein-coupled receptor E1 (F4/80) Function and Localization

• Adhesion G protein-coupled receptor E1 (gene Adgre1), also known as EMR1 or F4/80, is a single‑pass transmembrane glycoprotein belonging to the adhesion G protein‑coupled receptor family and characterized by the presence of EGF‑like domains.
• It functions as an orphan receptor involved in cell adhesion and immune cell‑cell interactions, with a suggested role in regulatory T‑cell (Treg) development.
• The receptor is predominantly expressed on the surface of mature macrophages; however, it is absent from macrophages located within T‑cell areas of lymph nodes and spleen, and is found at low levels on blood monocytes.
• Subcellularly, F4/80 localizes to the cell membrane, consistent with its roles in adhesion and intercellular communication.
• The extracellular region contains multiple EGF‑like repeats and mucin‑like domains, which are heavily glycosylated, contributing to its structural stability and ligand‑binding potential.
• Sequence analysis indicates the presence of disulfide bonds within EGF domains and a conserved GPCR autoproteolysis‑inducing domain, though no protease‑activated signaling has been reported.
• Due to its restricted expression on macrophages, F4/80 serves as a valuable marker for identifying and characterizing macrophage populations in the mouse immune system.
• The receptor is classified under immune‑related keywords such as adaptive immunity, glycoprotein, and G protein‑coupled receptor, underscoring its specialized role in immune cell biology.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein representing mouse F4/80; users may consider verifying specificity by comparing reactivity with F4/80‑deficient cell models or knockout mouse tissues.
• This APC‑conjugated antibody is validated for flow cytometry; for optimal staining, titrate the antibody using the relevant cell population and include appropriate isotype controls.
• In multicolor flow cytometry panels, ensure careful compensation, particularly when combining with other APC‑compatible fluorophores or detectors.
• Given the expression profile of F4/80 on macrophages, typical positive controls include thioglycollate‑elicited peritoneal macrophages or bone marrow‑derived macrophages; primary mouse blood monocytes express low levels and may serve as a dim control.
• For fixation and permeabilization protocols, assess whether antigenicity is preserved; freshly isolated cells are preferred to maintain surface marker integrity.

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