APC Rat anti-Mouse CD86 Monoclonal Antibody for Flow Cytometry - P42082

Core Product Specifications and Parameters

Biological Background: CD86 Function and Localization

• CD86 (B7-2) is a transmembrane glycoprotein and member of the immunoglobulin superfamily, acting as a costimulatory receptor for T-cell activation.
• It serves as a ligand for CD28, providing the primary auxiliary signal that augments MHC/TCR signaling in naïve T-cells, critical for immune response vs. anergy decisions within 24 hours (By similarity).
• CD86 also regulates B-cell function and IgG1 production; upon CD40 engagement, it activates NF-kappa-B via phospholipase C and protein kinase C. Related references: PMID:23241883
• Additionally, CD86 acts as an inhibitor of T-cell activation by serving as a ligand for CTLA4, a decoy receptor that blocks CD28-mediated priming. Related references: PMID:7504059, PMID:7513726
• It is alternatively spliced and contains immunoglobulin domains, disulfide bonds, and sites for N-linked glycosylation and ubiquitin conjugation.
• Expressed on the cell membrane of antigen-presenting cells, including dendritic cells, macrophages, and B cells.

Experimental Guidance and Technical Tips

• The APC conjugate enables direct detection by flow cytometry without a secondary antibody; titrate the antibody for your specific cell population to minimize background.
• CD86 is expressed on antigen-presenting cells; include appropriate isotype controls and consider Fc receptor blocking to ensure staining specificity.
• Validate the antibody in the relevant mouse strain and cell type, as CD86 expression levels can vary with activation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this high-quality anti-mouse CD86 antibody as part of a broad portfolio of immunological reagents for research and IVD development. As a one-stop sourcing partner, we offer validated monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents—backed by technical support from concept to clinic. For bulk orders or custom procurement of rare targets like CD86, contact our team to streamline your supply chain.