APC Rat Anti-Mouse CD45R/B220 Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45R/B220 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA), lymphocyte antigen 5 (Ly-5), T200, and B220, is a receptor-type tyrosine-protein phosphatase C encoded by the Ptprc gene (UniProt P06800).
• It is essential for T-cell activation through the antigen receptor, acting as a positive regulator of coactivation upon binding to DPP4.
• The first of its two intracellular phosphatase domains is enzymatically active, while the second influences substrate specificity.
• CD45 dephosphorylates key signaling molecules including LYN, SKAP1, and FYN, thereby modulating T-cell signaling thresholds.
• The protein localizes to the cell membrane, membrane rafts, and immunological synapse, facilitating its role in cell contact events.
• Interaction with CLEC10A on dendritic cells helps limit autoreactivity by modulating T-cell activation.
• CD45 undergoes alternative splicing, producing isoforms such as B220, and is subject to glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• This APC-conjugated antibody is designed for flow cytometry (FC). Consider titrating the antibody to determine the optimal concentration for your specific experimental setup.
• Include appropriate controls, such as an isotype control and unstained cells, to assess nonspecific binding and autofluorescence.
• The immunogen is a recombinant protein, suggesting recognition of a specific epitope; validate staining specificity in the relevant mouse lymphoid cell population (e.g., B cells expressing CD45R/B220).
• CD45R/B220 is commonly used as a B cell marker in mice; confirm expression patterns using co-staining with other lineage markers.

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CamelBio offers a streamlined one-stop sourcing solution for IVD raw materials, from monoclonal antibodies like this CD45R/B220 antibody to bulk ancillary reagents. Whether you need validated antibody pairs, optimized conjugates, or rare target raw materials for immunology and diagnostics, our team supports your product development pipeline from concept to clinic. By partnering with CamelBio, you gain access to a reliable supply chain that accelerates your assay development for leukocyte biology and T-cell activation research.