APC Rabbit anti-Mouse/Human CD55 Monoclonal Antibody for FC - Q61475

Core Product Specifications and Parameters

Biological Background: Complement Decay-Accelerating Factor (CD55) Function and Localization

• CD55, also known as decay-accelerating factor (DAF), is a GPI-anchored membrane protein that protects host cells from complement-mediated lysis by inhibiting the C3 and C5 convertases of both the classical and alternative pathways.
• The protein consists of four N-terminal short consensus repeats (SCRs or sushi domains), a heavily O-glycosylated serine/threonine-rich region, and a C-terminal GPI anchor that tethers it to lipid rafts on the cell membrane.
• By binding to C4b and C3b fragments deposited on cell surfaces, CD55 accelerates the decay of the C4b2a and C3bBb convertase complexes, thereby preventing the amplification of the complement cascade and protecting self-tissues from autoimmune attack.
• In mice, CD55 is expressed in a wide range of tissues, including brain, secretory epithelia, skeletal muscle, liver, testes, thymus, spleen, and lymph nodes, reflecting its broad role in innate immune surveillance.
• The CD55 gene belongs to the regulators of complement activation (RCA) cluster, and its protein product is structurally related to other complement regulatory proteins such as CD46 and CD35.
• Post-translational modifications include N-linked glycosylation in the SCR domains, extensive O-linked glycosylation in the mucin-like stalk, and addition of the GPI anchor; multiple disulfide bonds stabilize the sushi domain folds.

Experimental Guidance and Technical Tips

• This APC-conjugated monoclonal antibody is suitable for direct flow cytometry analysis of CD55 expression on live, unfixed cells; appropriate isotype controls and Fc receptor blocking should be included.
• The immunogen spans the majority of the extracellular domain (aa 35–361) of mouse CD55, ensuring recognition of native and recombinant forms; consider cross-reactivity validation with human samples due to sequence homology.
• Since CD55 is a GPI-anchored protein, avoid harsh permeabilization steps that may disrupt membrane microdomains; titration of the antibody is recommended to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For complement cascade and innate immunity research, this APC anti-CD55 monoclonal antibody is available along with full technical support and rapid global logistics. Leverage CamelBio’s sourcing expertise to accelerate your assay development from concept to clinic.