APC Rabbit anti-Mouse Ly-6C Monoclonal Antibody for Flow Cytometry - P0CW02

Core Product Specifications and Parameters

Biological Background: Lymphocyte antigen 6C1 Function and Localization

• Lymphocyte antigen 6C1 (Ly-6C1) is encoded by the Ly6c1 gene and is a cell surface glycoprotein anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage.
• As a member of the Ly-6/uPAR superfamily, Ly-6C features a conserved LU domain with multiple disulfide bonds that stabilize its three-dimensional structure.
• The protein is synthesized with a signal peptide, which is cleaved during maturation, and the GPI anchor is attached post-translationally at the C-terminus.
• Ly-6C localizes exclusively to the cell membrane, making it accessible for antibody-based detection methods such as flow cytometry.
• Keywords from UniProt indicate that Ly-6C is a glycoprotein, lipoprotein, and disulfide bond-containing membrane protein, underscoring its post-translational modification profile.
• Ly6c1 is part of the mouse reference proteome, confirming its high-quality annotation and importance as a canonical marker in mus musculus biology.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide spanning amino acids 1-100 of mouse Ly-6C (NP_001238984.1), which covers the N-terminal extracellular region containing the LU domain; this design typically yields antibodies that recognize the native protein on live cells.
• This APC-conjugated monoclonal antibody is already labeled with a bright fluorophore and is ready for direct immunofluorescence staining; no secondary antibody is needed, but appropriate isotype controls and Fc receptor blocking may be considered to minimize non-specific binding.
• For flow cytometry, consider titrating the antibody to determine optimal staining concentrations in your specific cell system, and validate staining patterns using known Ly-6C-positive and negative mouse immune cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, ancillary reagents, and customized procurement solutions. Whether you require bulk quantities of this anti-Ly-6C monoclonal antibody or need assistance sourcing rare-target reagents for mouse immunology research, CamelBio's supply chain expertise can support your development pipeline from concept to clinic.