APC Rabbit anti-Mouse IL1R1/CD121a Monoclonal Antibody for Flow Cytometry - P13504

Core Product Specifications and Parameters

Biological Background: IL1R1/CD121a Function and Localization

• Interleukin-1 receptor type 1 (IL1R1), also known as CD121a, is the primary receptor for the pro-inflammatory cytokines IL1A, IL1B, and the antagonist IL1RN.
• Upon ligand binding, IL1R1 associates with the co-receptor IL1RAP to form a high-affinity signaling complex, triggering NF-kappa-B and MAPK pathways downstream.
• Signaling is mediated through TIR domain interactions, recruiting adaptors TOLLIP, MYD88, IRAK1, or IRAK2, ultimately regulating inflammatory and immune responses.
• The receptor is a single-pass type I membrane protein, also found as a secreted form, and belongs to the immunoglobulin superfamily.
• Key post-translational modifications include N-linked glycosylation, phosphorylation, and disulfide bond formation, which are critical for proper folding and function.
• A brain-specific isoform (isoform 2) cooperates with IL1RAP isoform 3 to modulate IL1B-induced neuronal activity, including potentiation of NMDA receptor-mediated calcium influx via Akt kinase activation.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 20–338), making this antibody suitable for detecting surface-expressed IL1R1/CD121a on viable cells without permeabilization.
• As an APC conjugate, it is optimized for flow cytometry (FC). Titrate the antibody to determine the optimal staining concentration for your specific sample type and avoid over-saturation.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize nonspecific binding, especially when analyzing mouse myeloid or lymphocyte populations.
• Validate staining in the relevant mouse model system; positive expression is expected on T cells, macrophages, and certain neuronal cells.

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