APC Rabbit anti-Mouse IL-17A Monoclonal Antibody for Flow Cytometry (Intracellular) - Q62386

Core Product Specifications and Parameters

Biological Background: IL-17A Function and Localization

• Interleukin-17A (IL-17A), also known as CTLA-8, is a proinflammatory cytokine belonging to the IL-17 family and a signature effector of T-helper 17 (Th17) cells, playing a central role in antimicrobial host defense and tissue integrity. Related references: PMID:18025225 PMID:19144317 PMID:26431948
• Signals through the heterodimeric receptor IL17RA-IL17RC, recruiting TRAF3IP2 and activating TRAF6-dependent NF-κB and MAP kinase pathways to drive transcription of cytokines, chemokines, antimicrobial peptides, and matrix metalloproteinases, thereby orchestrating strong inflammatory responses. Related references: PMID:16200068 PMID:17911633 PMID:19144317
• Primarily induces neutrophil activation and recruitment at infection and inflammatory sites; in the airway epithelium, it mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. Related references: PMID:18025225 PMID:27923703
• In secondary lymphoid organs, IL-17A contributes to germinal center formation by modulating B cell chemotaxis to CXCL12 and CXCL13, enhancing B cell retention, somatic hypermutation, and plasma cell differentiation. Related references: PMID:18157131
• Functions as an effector cytokine of gamma‑delta T cells, iNKT cells, and group 3 innate lymphoid cells, promoting early neutrophilic inflammation and rapid bacterial clearance. Related references: PMID:17372004 PMID:20364087 PMID:28709803
• Plays a direct role in epithelial barrier integrity by regulating occludin (OCLN) localization and tight junction biogenesis upon acute injury; synergizes with IL‑17F to induce antimicrobial β‑defensins (DEFB1, DEFB103A, DEFB104A) from mucosal epithelial cells. Related references: PMID:26431948 PMID:19144317
• Contributes to antiviral host defense: enhances T cell cytotoxicity against West Nile virus, augments B cell responses in influenza A virus (H5N1) infection, and drives B‑1a cell differentiation for virus‑specific IgM production during H1N1 clearance. Related references: PMID:21946434 PMID:26735852 PMID:27795421
• Secreted glycoprotein with a molecular weight of ~17.5 kDa (precursor) and containing a signal peptide and one disulfide bond; expressed predominantly by Th17 cells and, to a lesser extent, by innate lymphoid cells, γδ T cell subsets, and iNKT cells.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, stimulate mouse T cells or relevant cell populations with PMA/ionomycin in the presence of a protein transport inhibitor (e.g., brefeldin A) for 4–6 hours prior to staining to enhance IL‑17A detection.
• After surface staining, fix and permeabilize cells using an appropriate fixation/permeabilization kit, and include a suitable isotype control and viability dye to monitor non‑specific binding and live/dead discrimination.
• Titrate the antibody to determine the optimal dilution for your specific cell type and activation conditions; clone‑to‑clone variability may influence binding affinity.
• Validate specificity in IL‑17A‑knockout mouse samples or using a blocking peptide/control antigen when available.
• Consider that the antibody was raised against amino acids 26–158, which encompasses the mature secreted form but lacks the signal peptide; confirm reactivity in the relevant biological context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This APC rabbit anti‑mouse IL‑17A monoclonal antibody is part of our broad portfolio supporting immunology and inflammation research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing to streamline your assay development and procurement needs.