APC Rabbit anti-Mouse IL-13 Monoclonal Antibody for Intracellular Flow Cytometry - P20109

Core Product Specifications and Parameters

Biological Background: IL-13 Function and Localization

• Interleukin-13 (IL-13), also known as T-cell activation protein P600, is a cytokine that plays critical roles in allergic inflammation and immune responses to parasitic infections (PubMed:15361238). Related references: PMID:15361238
• It synergizes with IL-2 to regulate interferon-gamma synthesis and stimulates the proliferation of B-cells, eosinophils, basophils, and mast cells.
• IL-13 controls IL-33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1 (IL1RL1) (PubMed:34789557). Related references: PMID:34789557
• The cytokine antagonizes Th1-driven proinflammatory immune responses and downregulates synthesis of many proinflammatory cytokines, including IL-1, IL-6, IL-10, IL-12, and TNF, partially via suppression of NF-kappa-B.
• On nonhematopoietic cells such as endothelial cells, IL-13 induces vascular cell adhesion protein 1 (VCAM1), important for eosinophil recruitment.
• IL-13 signals through a receptor complex comprising IL4R and IL13RA1, activating JAK1, TYK2, and STAT6 (PubMed:34795444, PubMed:8871614). Related references: PMID:34795444, PMID:8871614
• IL13RA2 acts as a high-affinity decoy receptor, mediating internalization and depletion of extracellular IL-13 (PubMed:29305434). Related references: PMID:29305434
• It induces inflammatory responses in esophageal epithelium, downregulating DSG1 and disrupting desmosome intercellular adhesion junctions (PubMed:24220297). Related references: PMID:24220297
• IL-13 is a secreted glycoprotein containing disulfide bonds (UniProt keywords: Secreted, Glycoprotein, Disulfide bond).

Experimental Guidance and Technical Tips

• The immunogen covers the mature mouse IL-13 sequence (amino acids 20-131), which lacks the signal peptide; this design targets the secreted form.
• As an APC-conjugated antibody, protect from light and avoid freeze-thaw cycles to maintain fluorophore stability.
• Intracellular flow cytometry (FC intra) requires cell permeabilization; consider optimizing fixation/permeabilization protocols for your cell type.
• While validated for intracellular flow cytometry, cross-reactivity with other species or suitability for other applications (e.g., ELISA, Western blot) should be experimentally verified.
• Validate antibody performance in your specific sample system, as expression levels of IL-13 may vary depending on cell stimulation or disease model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-IL-13 conjugate. For your allergy and immunology assay development, we can also source bulk ancillary reagents and rare target raw materials to streamline your workflow from concept to clinic. Partner with us for reliable, one-stop procurement tailored to your project's scale and technical needs.