APC Rabbit anti-Mouse IgG2b Monoclonal Antibody for FC - P01867-2

Core Product Specifications and Parameters

Biological Background: Mouse IgG2b Function and Localization

• Immunoglobulin heavy constant gamma 2B (Isoform 2) is a key component of mouse IgG2b antibodies, also known as Ig gamma-2B chain C region.
• Encoded by the Ighg2b gene (synonym Igh-3) with GeneID 16016.
• This protein represents the constant region of the IgG2b heavy chain, determining antibody isotype and effector functions.
• Subcellular localization is secreted, consistent with its role in humoral immunity.
• The predicted molecular weight is 36588 Da (UniProt); the antibody detects a band at approximately 37 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the IgG2b constant region (aa 14-113); confirm recognition of the native protein in your experimental system.
• Validated application: Flow cytometry (FC). Titrate the antibody to determine the optimal concentration for your specific cell population.
• Include appropriate isotype controls and Fc receptor blocking to reduce non-specific binding.
• The antibody is an APC conjugate; minimize light exposure to prevent fluorophore photobleaching.
• Assess cross-reactivity with other mouse immunoglobulin isotypes or species if multiplex staining is planned.
• For intracellular staining, a permeabilization step may be required; validate with positive and negative control samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects IVD developers with a comprehensive range of raw materials, including validated monoclonal antibodies for immunoglobulin targets. Our portfolio covers bulk quantities of anti-mouse IgG subclass reagents, optimized antibody pairs, and ancillary materials to streamline your assay development. Contact us to discuss sourcing for your next immunology or B cell research project.