APC Rabbit anti-Mouse ICOS/CD278 Monoclonal Antibody for FC - Q9WVS0

Core Product Specifications and Parameters

Biological Background: ICOS/CD278 Function and Localization

• Inducible T-cell costimulator (ICOS), also known as AILIM, CCLP, or CRP-1, is a transmembrane receptor belonging to the CD28/CTLA-4 family, characterized by an extracellular immunoglobulin domain.
• Acts as a stimulatory receptor on activated T-cells; binding to ICOSL on APCs delivers costimulatory signals that enhance T-cell proliferation, IL-10 secretion, upregulation of adhesion molecules, and provision of help for B-cell antibody production.
  Related references: PMID:10657606
• Critical for differentiation of T follicular regulatory cells during immune challenges such as viral infection.
  Related references: PMID:36754569
• Potentiates TCR-induced calcium flux through augmented PLCG1 activation and actin remodeling, and independently activates PI3K signaling.
  Related references: PMID:27693916, PMID:19915142
• Essential for efficient T‑B cell interaction, normal antibody responses to T‑cell dependent antigens, and prevention of apoptosis in pre‑activated T‑cells; mediates CD40-driven immunoglobulin isotype switching.
  Related references: PMID:11343122
• Predominantly localizes to the cell membrane as a single‑pass type I glycoprotein, featuring a disulfide‑bonded Ig‑like domain, a transmembrane helix, and potential N‑glycosylation sites.
• Expressed on activated T‑cells and resting memory T‑cells, with high levels in thymic medulla, lymph node germinal centers, and Peyer’s patches; low expression in spleen.

Experimental Guidance and Technical Tips

• The immunogen (aa 21–142) covers the extracellular Ig‑like domain of mouse ICOS, making this monoclonal antibody suitable for detecting surface‑exposed epitopes by flow cytometry (FC).
• As an APC‑conjugated reagent, it enables direct single‑step staining; titrate the antibody per cell number and check optimal concentration using activated murine splenocytes or relevant T‑cell lines.
• For cross‑reaction or multi‑species experiments, validate reactivity in the intended sample system; the antibody is reported to react with mouse ICOS.
• Combine with appropriate viability dyes and Fc‑block to minimize nonspecific binding, and include fluorescence‑minus‑one (FMO) controls for accurate gating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing. This APC‑conjugated anti‑mouse ICOS monoclonal antibody supports T‑cell costimulation and immunology workflows, exemplifying our commitment to furnishing high‑quality tools for every stage of assay development. Whether you need scale‑up quantities or custom formulation, rely on CamelBio as your single procurement partner.