APC Rabbit anti-Mouse CD71 Monoclonal Antibody for FC - Q62351

Core Product Specifications and Parameters

Biological Background: Transferrin receptor protein 1 Function and Localization

• Transferrin receptor protein 1 (TfR1, CD71) is a key membrane receptor mediating cellular iron uptake via receptor-mediated endocytosis of transferrin-bound iron.
• It is essential for the development of erythrocytes and the nervous system, enabling proper iron delivery for heme synthesis and neuronal function.
• Upon stimulation, TfR1 positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Related references: PMID:26642240
• TfR1 acts as a lipid sensor that modulates mitochondrial fusion via the JNK pathway; low dietary stearate triggers JNK-mediated MFN2 degradation, inhibiting fusion, while high stearate leads to TfR1 stearoylation, preventing JNK activation and preserving MFN2.
• It mediates the uptake of NICOL1 into fibroblasts, potentially influencing extracellular matrix production (PubMed:38625739). Related references: PMID:38625739
• Subcellularly, TfR1 localizes to the cell membrane and melanosomes, consistent with its role in endocytosis and trafficking.
• Post-translational modifications include disulfide bonds, glycosylation, palmitoylation, phosphorylation, and ubiquitin conjugation, which regulate its stability, localization, and function.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to APC, enabling straightforward flow cytometry without a secondary antibody. Optimize the dilution using a titration experiment in the target mouse sample.
• Use appropriate isotype controls and Fc receptor blocking to ensure specific detection of CD71 on mouse cells.
• For intracellular staining, consider permeabilization protocols that preserve surface epitopes if simultaneous membrane staining is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting your CD71-related research with ready-to-use antibodies and customized sourcing solutions. This APC Rabbit anti-Mouse CD71 monoclonal antibody is part of our portfolio for iron metabolism and immunology applications. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and specialized procurement for rare targets, accelerating your diagnostic development from concept to clinic.