APC Rabbit anti-Mouse CD59a Monoclonal Antibody for FC - O55186

Core Product Specifications and Parameters

Biological Background: CD59A glycoprotein Function and Localization

• CD59a is a potent inhibitor of the complement membrane attack complex (MAC), protecting self-cells from complement-mediated lysis.
• It binds to C8 components (C8A and C8B) of the assembling MAC and prevents incorporation of multiple C9 copies, blocking formation of the osmolytic pore.
• The protein is anchored to the cell membrane via a GPI anchor and is also secreted.
• It is broadly expressed across many tissues, including liver, kidney, spleen, thymus, brain, and heart, as well as in mononuclear cells, erythrocytes, and platelets, but barely detected in neutrophils.
• CD59a undergoes post-translational modifications including disulfide bond formation, glycosylation, and GPI-anchor attachment; it contains a signal peptide.
• Alternative names include MAC-inhibitory protein (MAC-IP), membrane attack complex inhibition factor (MACIF), and protectin.

Experimental Guidance and Technical Tips

• The APC-conjugated format is optimized for direct detection in flow cytometry; titrate the antibody to determine the optimal staining concentration for your specific sample.
• The immunogen corresponds to the extracellular domain (amino acids 24–96), suggesting that the antibody recognizes the native cell-surface protein; validate in appropriate mouse cell lines or primary cells.
• Given its broad tissue expression, confirm staining patterns with relevant biological controls (e.g., knockout or isotype controls) and viability dyes to exclude dead cells.
• For intracellular or secreted forms, permeabilization or alternative detection strategies may be required.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio supports diagnostic developers with a comprehensive portfolio of antibodies, antigens, and ancillary reagents. This anti-CD59a monoclonal antibody is well-suited for complement research and immune function studies, and we can assist with bulk orders, custom conjugations, or sourcing of additional components for your assay development pipeline.