APC Rabbit anti-Mouse CD47 mAb - Q61735

Core Product Specifications and Parameters

Biological Background: CD47 Function and Localization

• CD47, also known as integrin-associated protein (IAP), is a multifunctional cell surface receptor that contains an immunoglobulin domain and belongs to the immunoglobulin superfamily.
• It functions as an adhesive protein mediating cell-to-cell interactions and serves as a receptor for thrombospondin-1 (THBS1), modulating integrin signaling through heterotrimeric G proteins.
• CD47 plays critical roles in various physiological processes including inflammation, apoptosis, angiogenesis, cardiovascular homeostasis, and immunoregulation. Related references: PMID:20610415 PMID:23591719 PMID:27742621
• CD47 interaction with SIRPA prevents phagocytosis of red blood cells and inhibits dendritic cell maturation. Related references: PMID:10856220
• It also interacts with SIRPG to mediate T-cell adhesion and co-stimulation, and positively modulates FAS-dependent apoptosis in T-cells. Related references: PMID:15917238
• CD47 regulates stem cell self-renewal by inhibiting the expression of pluripotency factors SOX2, OCT4, MYC, and KLF4 in response to THBS1. Related references: PMID:23591719
• Subcellularly, CD47 is localized at the cell membrane and undergoes alternative splicing, generating multiple isoforms. Post-translational modifications include signal peptide cleavage, disulfide bond formation, glycosylation, and phosphorylation.
• Additionally, CD47 is involved in nitric oxide-mediated blood pressure regulation and negatively modulates wound healing. Related references: PMID:20610415 PMID:18156939

Experimental Guidance and Technical Tips

• The immunogen, encompassing the extracellular domain (aa 19-135), is expected to target the exposed region of CD47, making it suitable for detecting cell surface expression in flow cytometry.
• For flow cytometry, consider using appropriate isotype controls and titrating the antibody to optimize staining conditions.
• As CD47 is expressed on various hematopoietic and non-hematopoietic cells, validate specificity in the relevant cell type, particularly in blood cells and established cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of high-quality antibodies and recombinant proteins, including this anti-CD47 monoclonal antibody. Our platform simplifies procurement by offering validated antibody pairs, optimized detection reagents, and bulk ancillary materials for assays targeting immune checkpoint and cell adhesion pathways. Whether you need ready-to-use raw materials or customized sourcing for rare targets, CamelBio streamlines your transition from concept to clinic.