APC-Rabbit anti-Mouse CD44 Monoclonal Antibody for Flow Cytometry - P15379

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 is a cell-surface receptor also known as Ly-24, Pgp-1, HERMES, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, and hyaluronate receptor, encoded by the Cd44 gene (Gene ID: 12505) in mouse.
• It mediates cell-cell interactions, adhesion, and migration by binding extracellular matrix components such as hyaluronan, collagen, growth factors, cytokines, and proteases, and transduces signals via assembled protein complexes. Related references: PMID:25065622 PMID:8343954
• CD44 participates in lymphocyte activation, recirculation, and homing, as well as hematopoiesis and inflammation, contributing to immune responses and tissue microenvironment sensing.
• Upon interaction with LGALS9, CD44 activates LCK, ERK, and MAPK signaling pathways to promote natural killer cell activation. Related references: PMID:37006235
• The protein is localized to the cell membrane, microvilli, and can be secreted, with expression detected in the intestinal epithelium.
• It undergoes alternative splicing, glycosylation, phosphorylation, sulfation, and proteoglycan modification, and contains disulfide bonds, a signal peptide, and a transmembrane helix.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal dilution for your specific cell type and experimental conditions.
• Include appropriate isotype controls and viability dyes to ensure accurate gating and minimize non-specific binding.
• Validate the antibody in your sample system, as CD44 expression levels can vary with cell activation state and tissue type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop sourcing partner for IVD raw materials, offering validated monoclonal antibodies such as this CD44 antibody for diagnostic and research applications. Our comprehensive portfolio includes optimized antibody pairs, bulk ancillary reagents, and custom raw-material sourcing for rare targets, supporting your projects from concept to clinic. With technical services and consulting, we streamline your journey in immuno-oncology and cell adhesion research.