APC Rabbit anti-Mouse CD4 Monoclonal Antibody for FC - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 (T-cell surface glycoprotein CD4), also known as L3T4 or Ly-4, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily.
• In T-helper cells, CD4 serves as a coreceptor for MHC class II molecules, binding invariant regions of MHC-II while the T-cell receptor (TCR) recognizes the peptide antigen, thereby enhancing T-cell sensitivity and activation.
• Upon co-engagement with MHC-II, CD4 recruits the Src family kinase LCK to the TCR-CD3 complex, triggering phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) and downstream signaling cascades that promote lymphokine production, T-cell motility, and adhesion.
• CD4 is essential for the positive selection of T-helper cells in the thymus and also contributes to the differentiation of monocytes into mature macrophages.
• Beyond T cells, CD4 is expressed on other immune cells, including macrophages, dendritic cells, and NK cells, where it modulates differentiation, activation, cytokine expression, and cell migration through TCR/LCK-independent mechanisms.
• The protein contains an N-terminal signal peptide, four extracellular immunoglobulin-like domains (D1–D4), a transmembrane helix, and a short cytoplasmic tail with palmitoylation sites; it is N-glycosylated and stabilized by disulfide bonds.
• Alternative splicing generates multiple isoforms, with the canonical isoform having a calculated molecular mass of approximately 51 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 27–394) of mouse CD4, making this APC-conjugated antibody suitable for surface staining and flow cytometric analysis of live cells.
• For flow cytometry, titrate the antibody to an optimal concentration using the relevant cell population (e.g., mouse splenocytes or thymocytes) and include appropriate isotype controls and viability dyes.
• This antibody is specific for mouse CD4; its reactivity with other species has not been determined. Verify cross-reactivity if needed.
• The APC conjugate is compatible with standard flow cytometry instruments equipped with a red (633 nm) laser; avoid excessive light exposure to prevent photobleaching.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated primary antibodies, antibody pairs, and ancillary reagents. This APC anti-mouse CD4 monoclonal antibody is ideal for T-cell immunophenotyping applications in murine models, and we can assist with bulk orders, custom conjugations, and technical consultation. For rare-target sourcing or development of complete immunoassay systems, our team provides end-to-end support from concept to clinic.