APC Rabbit anti-Mouse CD38 Monoclonal Antibody for Flow Cytometry - P56528

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1) is a multifunctional transmembrane glycoprotein also known as I-19, ADPRC 1, and NIM-R5 antigen.
• It synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion, and possesses cADPR hydrolase activity.
• CD38 synthesizes the calcium mobilizer nicotinate-adenine dinucleotide phosphate (NAADP+) from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and 7.4, it preferentially transforms 2'-phospho-cADPR into NAADP+. Related references: PMID:11829748
• As a receptor, CD38 binds CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and migration across the endothelial barrier.
• In lamina propria T lymphocytes, CD38/CD31 interactions initiate signaling via LCK and LAT, leading to cytokine release. It also regulates dendritic cell functions including CCL21-driven migration, survival, and Th1 polarization.
• CD38 is localized to the membrane and features a signal-anchor, a transmembrane helix, disulfide bonds, and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 45–304, which encompasses the extracellular domain of mouse CD38, making this antibody suitable for flow cytometry detection of surface CD38.
• For flow cytometry, consider titrating the antibody on relevant mouse cell lines or primary cells to determine the optimal staining concentration.
• Verify reactivity and specificity in the appropriate mouse strain or tissue system, and include proper isotype controls and fluorescence compensation in multicolor panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to supply high-quality IVD raw materials, including validated monoclonal antibodies and ancillary reagents. Whether you need optimized anti-CD38 antibodies for immunology assays or rare-target sourcing, we provide reliable one-stop access from concept to clinic. Contact us to discuss your CD38-related research and procurement requirements.