APC Rabbit anti-Mouse CD34 Monoclonal Antibody for Flow Cytometry - Q64314

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34, also known as hematopoietic progenitor cell antigen CD34, is a transmembrane glycoprotein serving as a canonical marker for hematopoietic stem and progenitor cells.
• It may function as an adhesion molecule, mediating attachment of stem cells to the bone marrow extracellular matrix or stromal cells, and presenting carbohydrate ligands to selectins.
• The Cd34 gene undergoes alternative splicing, producing multiple isoforms.
• CD34 is localized at the membrane.
• Tissue expression includes the kidney (thin limb of Henle's loop) at the protein level, high expression in hematopoietic progenitor cell lines, brain, and testis, and moderate in thymus, spleen, and bone marrow; not detected in adult liver. Related references: PMID:31605441 PMID:1709048
• Post-translational modifications include glycosylation and phosphorylation.
• Its calculated molecular weight is approximately 41 kDa (UniProt: 40,983 Da).

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region of CD34 (aa 35-287), making this antibody suitable for detecting native surface-expressed antigen by flow cytometry.
• For flow cytometry, consider titrating the antibody concentration to determine optimal staining index for your target cell type.
• Ensure appropriate isotype controls are included for accurate gating.
• Validate in relevant mouse hematopoietic tissues (bone marrow, spleen) based on tissue expression pattern.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and researchers in hematopoiesis and stem cell biology. We offer a one-stop solution encompassing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target raw materials—streamlining your path from concept to clinic.