APC Rabbit anti-Mouse CD32 Monoclonal Antibody for Flow Cytometry (FC) - P08101

Core Product Specifications and Parameters

Biological Background: CD32 Function and Localization

• Low affinity immunoglobulin gamma Fc region receptor II (FcγRIIB), also known as CD32, encoded by the Fcgr2b gene in mouse. Alternative splicing produces multiple isoforms with distinct functional properties.
• Binds the Fc region of complexed immunoglobulins gamma with low affinity, mediating effector and regulatory functions such as phagocytosis of antigen-antibody complexes and modulation of antibody production by B-cells.
• Isoforms IIB1 and IIB1' form caps but fail to mediate endocytosis or phagocytosis, while isoform IIB2 can mediate endocytosis of soluble immune complexes via clathrin-coated pits.
• Coaggregation with B-cell receptors (BCR), T-cell receptors (TCR), or Fc receptors down-regulates activation of B-cells, T-cells, and mast cells, respectively.
• Located at the cell membrane, also found in the cytoplasm, associated with the cytoskeleton, and a secreted isoform (IIB3) is released by macrophages into serum.
• Widely expressed in hematopoietic cells; isoform IIB1 is preferentially expressed in lymphoid lineage, IIB2 in myeloid lineage, and IIB3 is serum-soluble.
• Post-translational modifications include glycosylation, phosphorylation, disulfide bonds, and the presence of a signal peptide and transmembrane domain; belongs to the immunoglobulin superfamily.

Experimental Guidance and Technical Tips

• As a monoclonal antibody, consistent lot-to-lot performance is expected. Titrate the antibody to determine the optimal working dilution for your specific cell type and experimental conditions.
• For flow cytometry, include appropriate Fc receptor blocking steps and isotype controls to minimize non-specific binding, especially when using cells with high Fc receptor expression.
• The APC conjugate offers bright fluorescence suitable for multicolor panels; ensure proper compensation controls are included when combining with other fluorophores.
• CD32 isoforms are differentially expressed; if isoform-specific detection is required, validate in your model system using literature or alternative methods.
• Cell surface staining with this antibody may require optimization of fixation and permeabilization protocols for combined intracellular staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This APC-conjugated anti-mouse CD32 antibody supports flow cytometric analysis of FcγRIIB in immunology, inflammation, and hematopoiesis studies. Our streamlined procurement platform bridges the gap from concept to clinic, ensuring reliable supply for your IVD development and research needs.