APC Rabbit anti-Mouse CD317/BST2 Monoclonal Antibody for FC - Q8R2Q8

Core Product Specifications and Parameters

Biological Background: CD317/BST2 Function and Localization

• CD317/BST2 (bone marrow stromal antigen 2), also known as HM1.24 antigen, is a 19 kDa protein encoded by the Bst2 gene in mouse (Gene ID: 69550).
• It functions as an interferon-induced antiviral host restriction factor, blocking release of diverse enveloped viruses (e.g., HIV-1, MMTV, MLV, EBOV, LASV, VSV) by directly tethering nascent virions to cell membranes.
• The protein acts as a physical tether, linking virions to the cell surface and to each other, leading to virus internalization and degradation or surface retention, thus restricting cell-free spread.
• CD317/BST2 also regulates cell growth and migration by inhibiting cell surface proteolytic activity of MMP14, which in turn decreases MMP15 activation.
• In plasmacytoid dendritic cells, it stimulates LILRA4/ILT7 signaling, providing negative feedback to type I interferon production during viral infection.
• It plays a role in organizing the subapical actin cytoskeleton in polarized epithelial cells.
• Subcellularly, CD317/BST2 localizes to the Golgi apparatus, trans-Golgi network, cell membrane, late endosomes, membrane rafts, cytoplasm, and apical cell membrane.
• The protein is specifically expressed on type I interferon-producing cells in naive mice, and features a GPI-anchor, coiled coil domains, disulfide bonds, and glycosylation.

Experimental Guidance and Technical Tips

• This APC-conjugated antibody is designed for flow cytometry; consider direct detection of surface CD317 on live mouse cells without secondary reagents.
• Validate staining in relevant sample systems, such as plasmacytoid dendritic cells or interferon-treated cell lines, as CD317 expression is IFN-inducible.
• For intracellular staining, include appropriate permeabilization steps, as CD317 localizes to multiple membrane compartments.
• When co-staining with other fluorophores, ensure compensation controls, as APC signal can be bright.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-CD317/BST2 antibody supports antiviral innate immunity and dendritic cell research, aligning with CamelBio's commitment to supplying rare-target reagents. Partner with CamelBio for reliable access to critical raw materials, from concept to clinic.