APC Rabbit anti-Mouse CD304/Neuropilin-1 Monoclonal Antibody for Flow Cytometry - P97333

Core Product Specifications and Parameters

Biological Background: Neuropilin-1 Function and Localization

• Neuropilin-1 (Nrp1, CD304, A5 protein) is a single-pass transmembrane receptor expressed in the nervous system and developing vasculature.
• Functions as a co-receptor for class 3 semaphorins and vascular endothelial growth factors (VEGFs), mediating chemorepulsion and angiogenesis (PubMed:26503042). Related references: PMID:26503042
• Binds semaphorin 3A (SEMA3A), PLGF-2, VEGF165, and VEGFB; co-expression with VEGFR2 (KDR) enhances VEGF165 binding and chemotaxis.
• Recognizes the C-end rule (CendR) motif R/KXXR/K on ligands, triggering cellular internalization and vascular leakage.
• Regulates cardiovascular development, neuronal circuit formation, and organogenesis; critical for motor neuron axon guidance and cell body migration (PubMed:26503042). Related references: PMID:26503042
• Subcellular localization includes the cell membrane, cytoplasm, and mitochondrial membrane.
• Contains multiple structural domains: CUB, coagulation factor domains, MAM, and a transmembrane helix; undergoes post-translational modifications including glycosylation, phosphorylation, and heparan sulfate attachment.
• Molecular weight: ~103 kDa; classified under developmental protein, receptor, and proteoglycan keywords.

Experimental Guidance and Technical Tips

• As the immunogen comprises nearly the entire extracellular domain (aa 22-856), the antibody likely recognizes native Neuropilin-1 on live cells, making it suitable for flow cytometry.
• For flow cytometry, titrate the antibody under your specific experimental conditions, and include appropriate isotype controls and viability dyes.
• Because Neuropilin-1 may be shed or secreted, consider analyzing both surface and intracellular fractions if required.
• Confirm specificity with knockout or knockdown models in your cell system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials. This APC-conjugated anti-Neuropilin-1 monoclonal antibody supports flow cytometry applications in angiogenesis and neurobiology research. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and offer rare-target sourcing to accelerate your assay development from concept to clinic.