APC Rabbit anti-Mouse CD24 Monoclonal Antibody for Flow Cytometry - P24807

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24 (Signal transducer CD24), also known as HSA, Ly-52, and nectadrin, is a glycosylphosphatidylinositol (GPI)-anchored cell membrane protein.
• May play a pivotal role in cell differentiation of various cell types, including early thymocyte development.
• Signaling is likely triggered by lectin-like ligand binding to CD24 carbohydrates, transduced by release of second messengers from the GPI-anchor.
• Modulates B-cell activation responses (By similarity).
• In association with SIGLEC10, may suppress immune responses to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. Related references: PMID:19264983
• Plays a role in the control of autoimmunity. Related references: PMID:20200274
• Subcellular location: Cell membrane, anchored via GPI. Predicted molecular weight approximately 8 kDa (UniProt: 7797 Da).
• Expressed in lymphoid, myeloid, and erythroid cells. Key biological processes include innate immunity and signal transduction.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell subset.
• Validate specificity using mouse CD24 knockout or knockdown cells, or by pre-incubating with the immunogen peptide.
• Include appropriate isotype controls and viability dyes to account for non-specific binding and dead cells.
• The antibody targets amino acids 27–53 of mouse CD24; confirm that this epitope is accessible under your experimental conditions (e.g., cell permeabilization may not be required for surface staining).
• As an APC conjugate, protect from light and use compatible filter sets (excitation ~650 nm, emission ~660 nm). Store at 4°C protected from light.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with validated antibody pairs, recombinant proteins, and bulk ancillary reagents. This APC-conjugated anti-CD24 monoclonal antibody supports flow cytometry analysis of mouse immune cell differentiation and B-cell activation. Leverage CamelBio's sourcing capabilities for rare targets and technical consultation—accelerating your IVD development from concept to clinic.