APC Rabbit anti-Mouse CD14 Monoclonal Antibody for FC - P10810

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein also known as myeloid cell-specific leucine-rich glycoprotein.
• Coreceptor for lipopolysaccharide (LPS): CD14 binds monomeric LPS in concert with LBP and delivers it to the LY96/TLR4 complex, initiating innate immune response. Related references: PMID:16148141
• Signal transduction: LPS-bound CD14 triggers signaling via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and inflammatory response. Related references: PMID:15895089 PMID:8612135
• Lipopeptide coreceptor: CD14 acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, with signaling from the cell surface and subsequent trafficking to Golgi in lipid rafts.
• M. tuberculosis lipoprotein receptor: CD14 serves as an accessory receptor for M.tuberculosis lipoproteins LprA, LprG and LpqH, in conjunction with TLR2 and TLR1, modulating antigen presenting cell functions. Related references: PMID:19362712
• Subcellular localization: CD14 is found on the cell membrane, anchored via GPI, and is also secreted. It localizes to membrane rafts and the Golgi apparatus upon activation.
• Tissue expression: CD14 is highly expressed on peritoneal macrophages, lung alveolar macrophages, dendritic macrophages, and lung macrophages. Related references: PMID:8612135 PMID:19362712
• Electronegative LDL binding: CD14 binds electronegative LDL (LDL(-)) and mediates cytokine release induced by LDL(-).

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 18-345), which is likely to generate antibodies recognizing the native conformation. Consider testing for cross-reactivity with other species if needed.
• Since the antibody is APC-conjugated, it is suitable for direct flow cytometry. Ensure proper compensation controls when using with other fluorophores.
• Validate the antibody in the relevant sample system (e.g., mouse peritoneal macrophages or lung macrophages) to confirm specificity.
• Because CD14 can be shed or secreted, consider appropriate gating strategies to distinguish membrane-bound from soluble forms if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This APC-conjugated anti-mouse CD14 monoclonal antibody supports immunology research and diagnostic development. For rare target raw-material sourcing or customized reagent needs, CamelBio ensures a seamless supply chain from concept to clinic.