APC Rabbit anti-Mouse CD124/IL-4Ra Monoclonal Antibody for Flow Cytometry - P16382

Core Product Specifications and Parameters

Biological Background: Interleukin-4 receptor subunit alpha Function and Localization

• Interleukin-4 receptor subunit alpha (IL-4Rα), also known as CD124, is the ligand-binding alpha chain of the type I and type II interleukin-4 receptors, which mediate the biological activities of interleukin-4 (IL-4) and interleukin-13 (IL-13).
• Upon ligand binding, IL-4Rα couples to the JAK1/2/3-STAT6 signaling pathway, leading to STAT6 phosphorylation and transcriptional regulation of genes involved in immune responses.
• IL-4Rα signaling promotes T helper type 2 (Th2) cell differentiation and is critical for the regulation of immunoglobulin E (IgE) production, as well as chemokine and mucus production at sites of allergic inflammation.
• In certain cell types, IL-4Rα can also signal through activation of insulin receptor substrates IRS1 and IRS2, linking cytokine signaling to metabolic pathways.
• The receptor is expressed in both Th1 and Th2 cells, indicating its broader role in immune cell communication beyond Th2 responses.
• Subcellularly, IL-4Rα is located at the cell membrane as a transmembrane protein, and a soluble secreted form may also be produced by alternative splicing.
• Keywords associated with IL-4Rα include alternative splicing, disulfide bond, glycoprotein, phosphoprotein, and receptor, reflecting its complex post-translational modifications and functional states.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific sample type, using an isotype control to assess non-specific binding.
• Validate the antibody’s reactivity in the relevant mouse strain and cell population, as expression levels of CD124 can vary.
• As an APC-conjugated antibody, protect from prolonged light exposure to maintain fluorophore stability and minimize photobleaching during sample preparation and acquisition.
• Consider appropriate cell viability markers and doublet discrimination gating to ensure accurate analysis of CD124 expression on live single cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of validated antibodies, including this APC-conjugated anti-mouse CD124 monoclonal antibody, to support immunoassay development and immunological research. Whether you require bulk ancillary reagents, rare target raw materials, or optimized antibody pairs, CamelBio streamlines procurement from concept to clinic. Explore how our supply capabilities can accelerate your diagnostic and research projects in cytokine signaling and allergic inflammation.