APC Rabbit anti-Mouse CD11b mAb - P05555

Core Product Specifications and Parameters

Biological Background: CD11b Function and Localization

• CD11b, or integrin alpha-M, combines with integrin beta-2 (CD18) to form the Mac-1 receptor, which is essential for various adhesive interactions of monocytes, macrophages, and granulocytes.
• It acts as the CR3 receptor, specifically binding the iC3b fragment of complement component C3, and likely recognizes the R-G-D peptide sequence in C3b.
• Beyond complement, CD11b serves as a receptor for fibrinogen and coagulation factor X, recognizing specific peptides in the fibrinogen gamma chain.
• CD11b plays a critical role in neutrophil migration and mediates adhesion between stimulated neutrophils and endothelial cells through interaction with ICAM1.
• In microglia, CD11b, together with TYROBP/DAP12, is required for the production of microglial superoxide ions, which promote neuronal apoptosis during brain development. Related references: PMID:18685038
• The protein is localized to the cell membrane and membrane rafts, and is predominantly expressed in monocytes, granulocytes, and subsets of peritoneal mast cells and microglia.
• CD11b is a glycoprotein that contains multiple domains with calcium and metal-binding sites, characteristic of integrin family adhesion receptors.

Experimental Guidance and Technical Tips

• This APC-conjugated antibody is designed for flow cytometry (FC). Perform titration experiments to determine the optimal concentration for your cell type and staining protocol to achieve clear resolution between positive and negative populations.
• The immunogen targets an extracellular region (amino acids 143-337) of mouse CD11b. Validate the antibody's reactivity in physiologically relevant mouse cell lines or primary cells, such as monocytes, macrophages, or granulocytes.
• When designing multicolor flow cytometry panels, include appropriate isotype controls, viability dyes, and compensation controls to ensure accurate data interpretation.
• As a monoclonal antibody, it offers high specificity and lot-to-lot consistency, but cross-reactivity with other species or isoforms should be independently tested if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare targets for immunology and cell adhesion studies. This APC Rabbit anti-Mouse CD11b mAb reflects our capability to deliver high-quality, application-specific antibodies that support your research and diagnostic assay development.