APC Rabbit anti-Mouse CD103 mAb for FC - Q60677

Core Product Specifications and Parameters

Biological Background: Integrin alpha-E Function and Localization

• Identified as Integrin alpha-E, also known as CD103, integrin alpha M290, alpha-E1, and aM290. It is encoded by the Itgae gene in mouse.
• Functions as a heterodimer with integrin beta-7 (ITGB7) to form the alpha-E/beta-7 integrin, serving as a receptor for E-cadherin.
• Mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers, playing a critical role in mucosal immune surveillance.
• Subcellular localization: Primarily a single-pass type I membrane protein, expressed on the cell surface.
• Molecular features: Contains extracellular integrin alpha domains with calcium- and metal-binding sites essential for ligand interaction; classified under integrin family, glycoprotein, and receptor keywords.
• The mature protein has a calculated molecular mass of approximately 129 kDa (UniProt: 128,906 Da), consistent with its glycosylation state.

Experimental Guidance and Technical Tips

• The antibody is APC-conjugated and specifically designed for flow cytometry (FC). Ensure proper laser and filter settings for APC detection (excitation: 633-635 nm; emission: 660 nm).
• Since the immunogen covers amino acids 191-385 (extracellular region), the antibody likely recognizes an extracellular epitope, suitable for live-cell surface staining.
• Validate the antibody's reactivity in your specific mouse tissue or cell type of interest, ideally with a known CD103-positive control (e.g., intraepithelial lymphocytes).
• For optimal performance, titrate the antibody concentration in your assay and use appropriate isotype controls.
• Note that the antibody is supplied as a monoclonal rabbit antibody, which may reduce lot-to-lot variability compared to polyclonal preparations.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized provider of IVD raw materials, CamelBio offers convenient access to validated monoclonal antibodies like this anti-CD103 clone, along with a comprehensive portfolio of ancillary reagents and bulk supply options. Whether you are developing mucosal immunology assays or exploring novel diagnostic targets, our one-stop platform streamlines procurement and technical consultation. We support research and diagnostic partners with reliable, high-quality materials tailored to their workflow needs.