APC Rabbit anti-Human/Mouse PDGFRα/CD140a Monoclonal Antibody for FC - P16234

Core Product Specifications and Parameters

Biological Background: PDGFRα Function and Localization

• Platelet-derived growth factor receptor alpha (PDGFRα) is a cell-surface tyrosine kinase receptor that binds PDGFA, PDGFB, and PDGFC, initiating signaling cascades that regulate cell proliferation, survival, chemotaxis, and differentiation. (UniProt: P16234)
• The receptor plays essential roles in embryonic development, including skeleton formation, cephalic closure, gastrointestinal mucosa development, and intestinal villi formation, and is required for the differentiation of bone marrow-derived mesenchymal stem cells.
• PDGFRα is encoded by the PDGFRA gene and is also known as CD140a, PDGFR2, and PDGFR-2; alternative splicing generates multiple isoforms, and the protein contains immunoglobulin-like domains in its extracellular region.
• Ligand-induced activation leads to phosphorylation of downstream effectors such as PIK3R1, PLCG1, and PTPN11, and promotes signaling through the AKT1, MAPK/ERK, and STAT pathways, modulating diverse cellular responses.
• Subcellularly, PDGFRα is localized to the cell membrane, as well as the Golgi apparatus and primary cilium, reflecting its dynamic trafficking and signaling roles.
• The receptor is widely expressed, with detection in platelets, brain, fibroblasts, smooth muscle, heart, and embryo, and is also found in primary and metastatic colon tumors and normal colon tissue.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination; the protein is classified as a proto-oncogene and is linked to various disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 24-524), making this antibody suitable for detecting surface PDGFRα in unfixed or fixed non-permeabilized cells by flow cytometry.
• For flow cytometry, titrate the APC conjugate to determine the optimal staining concentration and use appropriate isotype controls and Fc receptor blocking to minimize nonspecific binding.
• Since this antibody cross-reacts with mouse PDGFRα, validate the staining pattern in mouse cell lines or primary cells to confirm specificity in the intended model system.
• APC is a bright fluorochrome; ensure proper laser and filter configuration (e.g., 633-635 nm excitation, 660/20 emission) and include viability dyes to exclude dead cells that may bind antibody non-specifically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories by providing one-stop access to premium IVD raw materials. This APC-conjugated rabbit monoclonal antibody against PDGFRα/CD140a is part of our extensive catalog of validated antibodies, recombinant proteins, and ancillary reagents, supporting oncology, developmental biology, and stem cell research. Beyond antibody supply, we offer bulk reagent procurement, custom development, and technical consultation to streamline your assay development and scale-up needs.