APC Rabbit anti-Human/Monkey PD-1/CD279 mAb for Flow Cytometry - Q15116

Core Product Specifications and Parameters

Biological Background: PD-1/CD279 Function and Localization

• Programmed cell death protein 1 (PD-1, CD279) is an inhibitory receptor on antigen-activated T-cells essential for maintaining immune tolerance and preventing autoimmunity. Related references: PMID:21276005 PMID:31754127 PMID:32184441
• Upon engagement with its ligands PD-L1 (CD274) and PD-L2 (CD273), PD-1 transmits inhibitory signals that dampen T-cell receptor (TCR)-mediated activation. Related references: PMID:21276005 PMID:26602187
• Ligand binding induces phosphorylation of the ITSM motif, recruiting the phosphatase SHP-2 (PTPN11) which dephosphorylates key TCR proximal signaling components including ZAP70, PKCθ, and CD3ζ. Related references: PMID:32184441
• The PD-1 pathway is exploited by tumor cells to suppress anti-tumor immunity; interaction with PD-L1 on tumor cells inhibits cytotoxic T lymphocyte effector function, promoting immune escape. Related references: PMID:28951311
• Therapeutic blockade of the PD-1/PD-L1 axis reverses T-cell exhaustion and restores anti-tumor immune responses, providing a clinical rationale for cancer immunotherapy. Related references: PMID:22658127 PMID:25034862 PMID:25399552
• PD-1 is localized at the cell membrane and features an immunoglobulin-like domain, disulfide bonds, and post-translational modifications including glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the APC-conjugated antibody to determine the optimal staining concentration; start with 0.5–5 µg/mL and adjust based on signal-to-noise ratio.
• Use an appropriate APC-conjugated rabbit IgG isotype control to correct for nonspecific binding.
• Perform live-cell staining at 2–8°C to prevent PD-1 internalization and ensure maximal surface detection.
• The antibody cross-reacts with cynomolgus monkey PD-1, facilitating non-human primate studies. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering this anti-PD-1/CD279 antibody to support immuno-oncology and immune checkpoint research. Beyond antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials to accelerate your diagnostic development. Trust our global sourcing network to deliver rare or custom raw materials with the consistency and compliance required for IVD manufacturing.