APC Rabbit anti-Human/Monkey Ki67 Monoclonal Antibody for Intracellular Flow Cytometry - P46013

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Ki-67 acts as a chromosome repellent during early mitosis, preventing chromosome collapse by forming a steric and electrostatic charge barrier on the chromosome surface. Related references: PMID:27362226
• During mitotic anaphase, Ki-67 switches to a chromosome attractant, promoting chromosome clustering and excluding cytoplasmic particles from the future nuclear space. Related references: PMID:32879492 PMID:39153474
• Dephosphorylation during mitotic exit and exposure of a conserved basic patch induce RNA-dependent liquid-liquid phase separation, coalescing chromosome surfaces. Related references: PMID:39153474 PMID:28935370
• Ki-67 binds to DNA, with a preference for supercoiled and AT-rich DNA. Related references: PMID:10878551
• Subcellular localization includes chromosome surfaces, nucleoli, and the nucleus, consistent with its dynamic role during the cell cycle.
• The protein undergoes multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination, which likely regulate its activity and localization.
• Ki-67 may influence higher-order chromatin organization, though its direct role remains under investigation. Related references: PMID:24867636
• Alternative splicing generates multiple isoforms of Ki-67, potentially modulating its function across different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 2271–2370 near the C-terminus; the antibody may recognize both full-length and relevant spliced isoforms containing this epitope.
• For intracellular flow cytometry, optimize fixation and permeabilization steps to ensure accessibility of the nuclear Ki-67 antigen.
• Use an appropriate isotype control and validate in a proliferating cell line (e.g., HeLa) with a resting cell negative control to confirm specificity.
• Due to the large protein size (359 kDa), consider acquiring sufficient events and using appropriate fluorescence compensation settings.

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CamelBio streamlines IVD raw material procurement—from concept to clinic—by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-Ki67 antibody serves proliferation-based oncology and cellular health assays, and we can support your development with matched reagents and flexible supply solutions.