APC Rabbit anti-Human/Monkey IgM Monoclonal Antibody for Flow Cytometry - P01871

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Heavy Constant Mu Function and Localization

• The IGHM gene encodes the constant region of the mu heavy chain, which defines the IgM antibody isotype. IgM is the first antibody produced during humoral immunity, serving both as a membrane-bound B cell receptor (BCR) and a secreted effector molecule. Related references: PMID:20176268 PMID:22158414 PMID:17576170
• Secreted IgM (sIgM) multimerizes predominantly into pentamers via the Fc region, providing high-avidity antigen binding and enabling recognition of conserved self- and pathogen-derived structures. Related references: PMID:32029689 PMID:37095205
• sIgM mediates effector functions by activating the classical complement pathway through C1q binding and engaging Fc receptors, which promotes phagocytosis of apoptotic cells and induction of neutralizing IgG responses. Related references: PMID:12847249 PMID:19006321 PMID:28230186
• Membrane-bound IgM forms the BCR complex, providing constitutive tonic signaling essential for B cell survival and mediating pre‑BCR signaling that regulates B cell selection and allelic exclusion.
• IgM plays a critical role in mucosal immunity by undergoing transcytosis across mucosal epithelium via PIGR. IgM–antigen complexes are retrotranscytosed by FCMR across M cells for antigen presentation. Related references: PMID:32029689
• Subcellular localization is primarily at the cell membrane (as BCR) or secreted (as soluble antibody). Post‑translational modifications include N‑glycosylation and intra‑/inter‑chain disulfide bond formation.
• Alternative splicing generates membrane‑bound and secreted isoforms, allowing the same heavy chain gene to produce both receptor and antibody forms.

Experimental Guidance and Technical Tips

• The immunogen spans the IgM constant region (aa 218‑453), so the antibody is expected to recognize total human IgM regardless of antigen specificity. Confirm detection of both soluble and membrane‑bound IgM in your system.
• This APC‑conjugated antibody is suitable for direct flow cytometry without a secondary detection step. Include appropriate isotype controls and Fc‑blocking reagents to minimize background.
• Cross‑reactivity with Cynomolgus monkey IgM is indicated; verify performance if using other primate species.
• Since the protein weight of secreted IgM heavy chain is approximately 49 kDa, consider size‑based gating or confirmation by western blot under reducing conditions if assay validation requires it.
• For intracellular IgM staining, optimize fixation and permeabilization conditions for your specific cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated B2B partner for IVD raw‑material sourcing, offering a comprehensive portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. For IgM‑related immunoassay development, we provide one‑stop access to quality‑controlled antibodies and can assist with sourcing rare‑target raw materials tailored to your diagnostic pipeline, from early R&D through clinical application.