APC Rabbit anti-Human/Monkey CD8a mAb for FC - P01732

Core Product Specifications and Parameters

Biological Background: T-cell surface glycoprotein CD8 alpha chain Function and Localization

• CD8A encodes the alpha chain of the CD8 coreceptor, which exists as either a disulfide-linked alpha/beta heterodimer or an alpha/alpha homodimer; alternative splicing yields isoforms with calculated molecular weights of approximately 21, 25, and 30 kDa.
• As an integral membrane glycoprotein of the immunoglobulin superfamily, CD8A plays a pivotal role in adaptive immunity by functioning as a coreceptor for MHC class I molecules, interacting simultaneously with the T-cell receptor (TCR) and MHC I on antigen-presenting cells.
• Upon MHC I engagement, CD8A recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that promote lymphokine production, motility, adhesion, and cytotoxic T-lymphocyte (CTL) activation, enabling the elimination of infected or tumor cells.
• In natural killer (NK) cells, CD8A homodimers provide a survival mechanism that facilitates conjugation and lysis of multiple target cells, and also support the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
• CD8A is predominantly expressed on thymus-derived T-cells, but can also be found on subsets of NK cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells; notably, it is upregulated on the surface of plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.
• The protein is localized at the cell membrane (single-pass type I membrane protein) and is also secreted; it undergoes several post-translational modifications including disulfide bond formation, glycosylation, and palmitoylation.
• Keywords associated with CD8A include adaptive immunity, alternative splicing, glycoprotein, and disease variant, underscoring its critical role in immune defense and its relevance to immunodeficiencies.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers amino acids 22–182 of human CD8A, which corresponds to the extracellular domain; this design suggests recognition of the native cell-surface form, making it well suited for flow cytometry applications.
• For flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions; always include appropriate isotype controls.
• The reported cross-reactivity with cynomolgus monkey (Macaca fascicularis) indicates utility in non-human primate immunophenotyping studies; validation in the relevant sample system is advised.
• Because CD8A can be expressed as homodimers or heterodimers and has multiple reported molecular weights, confirm specificity in your target cell population using parallel markers or knockout controls if necessary.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio connects diagnostic manufacturers, laboratories, and researchers with critical reagents like this anti-CD8a monoclonal antibody to accelerate immunology and T-cell monitoring applications. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets, ensuring seamless integration into your assay development pipeline. From concept to clinic, CamelBio simplifies procurement so you can focus on delivering reliable diagnostic solutions.