APC Rabbit anti-Human/Monkey CD86 Monoclonal Antibody for FC - P42081

Core Product Specifications and Parameters

Biological Background: CD86 Function and Localization

• CD86 (also known as B7-2, B70, LAB72, CD28LG2) is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, characterized by an extracellular IgV-like domain and a cytoplasmic tail.
• CD86 acts as a costimulatory molecule for T‑lymphocyte activation by binding to the CD28 receptor, providing the primary auxiliary signal for naive T‑cell priming.
  Related references: PMID:12196291, PMID:7694363
• Within the first 24 hours after T‑cell activation, CD86 co‑stimulation is critical for determining immunity versus anergy.
  Related references: PMID:7527824
• CD86 also binds to CTLA‑4, a decoy receptor, thereby blocking CD28‑mediated priming and acting as a negative feedback inhibitor of T‑cell activation.
  Related references: PMID:11279501
• In B cells, CD86 engagement upon CD40 ligation activates the NF‑kappa‑B signaling pathway and regulates IgG1 production (by similarity).
• CD86 is expressed on the surface of professional antigen‑presenting cells, including dendritic cells, macrophages, and B cells.
• It serves as a host receptor for subgroup B adenoviruses, facilitating viral entry.
• Post‑translational modifications include glycosylation and ubiquitination; the gene undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 20‑241), suggesting the antibody recognizes native surface CD86; it is appropriate for detecting cell‑surface expression by flow cytometry.
• For flow cytometry, consider titrating the antibody and using appropriate isotype controls to determine optimal staining concentrations.
• The antibody is APC‑conjugated, enabling direct detection without a secondary reagent, which can reduce background and simplify multi‑color panel design.
• Cross‑reactivity with cynomolgus monkey has been confirmed; verify staining patterns in your target species and cell types experimentally.
• Cell surface staining protocols should include Fc receptor blocking to minimize non‑specific binding, especially for myeloid antigen‑presenting cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including this validated CD86 monoclonal antibody, to support immunology research and diagnostic development. As a one‑stop sourcing partner, we offer bulk reagents, validated antibody pairs, and customized solutions for rare‑target raw materials, enabling seamless transition from concept to clinical application.