APC Rabbit anti-Human/Monkey CD69 mAb for FC - Q07108

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 is a type II transmembrane glycoprotein belonging to the C-type lectin family, encoded by the CD69/CLEC2C gene; it is also known as AIM, EA1, MLR-3, GP32/28, BL-AC/P26, and Early T-cell activation antigen p60.
• Rapidly induced on the surface of T-cells, platelets, and NK cells upon activation, CD69 stimulates diverse signaling pathways to regulate immune cell homeostasis.
  Related references: PMID:24752896, PMID:26296369, PMID:35930205
• It modulates T-cell differentiation: CD69 negatively regulates Th17 development through carbohydrate-dependent interaction with galectin‑1 and JAK3/STAT5/RORC signaling, while also promoting conversion of naive CD4 T-cells into regulatory T-cells via the S100A8/S100A9 complex.
  Related references: PMID:24752896, PMID:26296369
• Acting as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T‑lymphocytes, CD69 suppresses inflammatory responses by inducing PDCD1 expression through NFAT activation.
  Related references: PMID:35930205
• CD69 contributes to host defense by directly recognizing P. aeruginosa, leading to ERK1 activation and GM‑CSF secretion; in eosinophils, it induces IL‑10 production through the ERK1/2 pathway.
  Related references: PMID:34841721
• CD69 negatively regulates chemotactic responses of effector lymphocytes and dendritic cells by acting as a S1PR1 agonist, promoting receptor internalization and degradation.
  Related references: PMID:37039481
• Localized at the cell membrane, CD69 is expressed on activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets.

Experimental Guidance and Technical Tips

• This APC-conjugated antibody is suitable for direct flow cytometry of surface CD69 on activated human and cynomolgus monkey lymphocytes; consider using appropriate isotype controls and Fc‑blocking reagents.
• The immunogen spans the extracellular domain (aa 78–199) of human CD69; verify epitope conservation and cross-reactivity in the relevant biological system.
• For multicolor panels, ensure correct compensation and avoid spectral overlap with the APC channel; confirm staining specificity by comparing stimulated versus unstimulated cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined, one-stop sourcing platform for IVD raw materials, including validated antibody pairs, bulk monoclonal/polyclonal antibodies, and accessory reagents. With deep expertise in immunology and immuno-oncology targets such as CD69, we accelerate assay development from concept to clinic. Let us support your next project with robust supply chain solutions.