APC Rabbit Anti-Human/Monkey CD45 Monoclonal Antibody for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as Leukocyte common antigen (L-CA) or T200, is a receptor-type tyrosine-protein phosphatase C encoded by the PTPRC gene. Multiple isoforms exist due to alternative splicing.
• It functions as a key positive regulator of T-cell activation, dephosphorylating signaling molecules like SKAP1, FYN, and LYN to modulate TCR signaling (PubMed:35767951). Related references: PMID:35767951
• CD45 interacts with CLEC10A at the immune synapse, which modulates T cell activation threshold to limit autoreactivity.
• It serves as a receptor for human cytomegalovirus protein UL11, mediating viral-induced T cell proliferation impairment.
• The protein localizes to the cell membrane, membrane rafts, and immunological synapse.
• Tissue-specific expression of isoforms is observed: isoforms 1,2,3,5,7 are detected in thymocytes, while isoforms 4,6,8 are not.
• Key post-translational modifications include glycosylation and phosphorylation. The canonical molecular weight is around 147 kDa, but isoform variants range from 131 to 147 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 26-577), making this antibody well-suited for live-cell flow cytometry. Consider validating performance in your specific FC panel with appropriate compensation controls.
• The antibody is documented to react with human and cynomolgus CD45. For other species or tissues, verify cross-reactivity experimentally.
• Due to the existence of multiple CD45 isoforms with varying molecular weights, confirm specificity in relevant cell types if isoform discrimination is needed.
• For best results, use freshly isolated cells and follow standard cold staining protocols to prevent antigen modulation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories a seamless route to validated IVD raw materials, including this APC-conjugated anti-CD45 monoclonal antibody. Our platform provides optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expedited rare-target raw-material sourcing to accelerate immunoassay development. Whether you need complete antibody pairs or custom formulations for immune monitoring, CamelBio bridges the gap from concept to clinic.