APC Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for FC - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 (T-cell surface glycoprotein, alias T4/Leu-3) is a member of the immunoglobulin superfamily, featuring four extracellular Ig-like domains, a single transmembrane helix, and a cytoplasmic tail that interacts with intracellular signaling molecules.
• Functions as a coreceptor for MHC class II molecules on antigen-presenting cells: upon antigen recognition, CD4 binds MHC class II and simultaneously associates with the T-cell receptor (TCR), recruiting the Src kinase LCK to phosphorylate CD3 chains and initiate T-cell activation, lymphokine production, and cellular adhesion.
• Essential for T-helper cell development in the thymus and for the maintenance of peripheral T-helper cell function; also triggers differentiation of monocytes into mature macrophages.
• Highly expressed on the surface of T-helper cells and, to a lesser extent, on other immune cells including macrophages, dendritic cells, and NK cells, where it modulates differentiation, cytokine expression, and migration.
• Localized primarily at the cell membrane as a single-pass type I membrane protein; internalization can be triggered upon ligand binding or viral interaction.
• Key post‑translational modifications include phosphorylation of the cytoplasmic tail, palmitoylation at a membrane-proximal cysteine, and N‑linked glycosylation of the extracellular domains, which are critical for membrane localization and protein interaction.
• Acts as a receptor for multiple viruses: it is the primary receptor for HIV-1, binding the viral envelope glycoprotein gp120 to mediate viral entry; is down-regulated by HIV-1 Vpu protein; and also serves as a receptor for Human Herpesvirus 7 (HHV-7). Related references: PMID:12089508 PMID:16331979 PMID:2214026 PMID:17346169 PMID:7909607

Experimental Guidance and Technical Tips

• This antibody is conjugated to Allophycocyanin (APC) and supplied in a format ready for flow cytometry (FC). The immunogen was recombinant human CD4 protein, and the antibody was generated in rabbit as a monoclonal source.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions. Use an appropriate isotype control conjugate to assess non‑specific binding.
• The antibody has been validated to cross‑react with human and cynomolgus monkey CD4. For other species, independent validation is recommended.
• Prepare single‑cell suspensions using standard protocols and include a viability dye to exclude dead cells for accurate immunophenotyping.
• For intracellular staining or combined surface/intracellular protocols, optimize fixation and permeabilization steps as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This APC anti-CD4 monoclonal antibody is well-suited for immunophenotyping and immune function studies in flow cytometry. Beyond this product, CamelBio offers a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and manufacturing needs.