APC Rabbit Anti-Human/Monkey CD3 Monoclonal Antibody for FC - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Epsilon Chain Function and Localization

• CD3 epsilon (CD3e) is a core component of the T-cell receptor (TCR)-CD3 complex on T-lymphocytes, playing a pivotal role in adaptive immunity (PubMed:15294938, PubMed:15546002, PubMed:2470098).
  Related references: PMID:15294938 PMID:15546002 PMID:2470098
• Upon antigen recognition, CD3e and other CD3 chains (CD3D, CD3G, CD247/CD3Z) transmit signals across the membrane by phosphorylating immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains, leading to ZAP70 recruitment and downstream signaling (PubMed:2470098, PubMed:40592325).
  Related references: PMID:2470098 PMID:40592325
• CD3e is critical for T-cell development and the internalization and down-regulation of TCR-CD3 complexes via endocytosis motifs in its cytoplasmic region (PubMed:10384095, PubMed:26507128).
  Related references: PMID:10384095 PMID:26507128
• Beyond its TCR coreceptor role, CD3e acts as a receptor for ITPRIPL1; ligand binding inhibits T-cell activation by promoting NCK1 interaction, blocking ERK-NFkB signaling and calcium influx (PubMed:12110186, PubMed:38614099).
  Related references: PMID:12110186 PMID:38614099
• CD3e is a single-pass type I membrane protein located at the cell membrane, containing an immunoglobulin-like domain and a long cytoplasmic tail with ITAMs.
• Post-translational modifications include phosphorylation on ITAM tyrosines, which are essential for signal transduction.
• Keywords: adaptive immunity, T-cell activation, co-receptor, phosphoprotein, transmembrane protein.

Experimental Guidance and Technical Tips

• This antibody is APC-conjugated; when using for flow cytometry, ensure your instrument is equipped with appropriate laser (e.g., 633/635 nm) and filter sets. Include compensation controls and titrate the antibody for optimal staining index.
• The original immunogen corresponds to amino acids 1-126 of human CD3 (extracellular domain), suggesting recognition of an exposed epitope. Performance has been reported with human and cynomolgus monkey samples; consider cross-reactivity screening for other primate species if needed.
• For T-cell immunophenotyping, this antibody can be combined with other lineage markers (e.g., CD4, CD8, CD45) in multicolor panels. Always validate in the relevant sample system (e.g., PBMCs, whole blood) with appropriate viability and Fc-blocking steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. We offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for immunology targets like CD3. Our sourcing network enables access to rare target raw materials and customized supply solutions.