APC Rabbit anti-Human/Monkey CD25 Monoclonal Antibody for Flow Cytometry - P01589

Core Product Specifications and Parameters

Biological Background: Interleukin-2 receptor subunit alpha Function and Localization

• IL2RA encodes the Interleukin-2 receptor subunit alpha, also known as TAC antigen, p55, or CD25.
• Functions as a receptor for interleukin-2 and is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity.
• TREGs suppress the activation and expansion of autoreactive T-cells, contributing to peripheral tolerance.
• Subcellular location: Membrane; single-pass type I membrane protein.
• Contains a signal peptide, sushi domains (Repeat), a transmembrane helix, and multiple disulfide bonds; it is a glycoprotein.
• Associated with diabetes mellitus and other immune-related disorders (disease variant).
• Molecular weight of the unprocessed protein is approximately 30.8 kDa (calculated), but glycosylation and other modifications result in an observed weight of ~31 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of CD25, making this antibody suitable for detection of surface-expressed CD25 on T-cells.
• Recommended for flow cytometry; consider titrating the antibody to optimize staining for specific cell types.
• Validated in human and cynomolgus monkey samples; cross-reactivity with other species should be tested if required.
• Since it is APC-conjugated, avoid freeze-thaw cycles and protect from light to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this APC-conjugated anti-CD25 monoclonal antibody as part of a comprehensive portfolio of IVD raw materials for immunology and immune monitoring. We provide validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your diagnostic and research workflows.