APC Rabbit anti-Human/Monkey CD20 mAb - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20 (MS4A1) is a B-lymphocyte-specific membrane protein, also designated B1, Bp35, and Leu-16, belonging to the membrane-spanning 4-domains subfamily A.
• It regulates calcium influx essential for B-cell development, differentiation, and activation, acting as a store-operated calcium channel component downstream of the B-cell receptor. Related references: PMID:12920111, PMID:3925015, PMID:7684739, PMID:18474602
• The human MS4A1 gene (GeneID: 931) encodes the protein, with multiple isoforms resulting from alternative splicing.
• CD20 localizes to the cell membrane; it is an integral transmembrane protein with four membrane-spanning domains.
• Its expression is restricted to B-lymphocytes, making it a key marker for B-cell lineage.
• The protein undergoes palmitoylation and phosphorylation, contains disulfide bonds, and is a pharmaceutical target (e.g., rituximab).
• The canonical and alternative isoforms exhibit molecular weights of approximately 33 kDa and 14 kDa, respectively.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an intracellular loop (aa 100–200); for live-cell surface staining, confirm epitope accessibility and consider fixation/permeabilization protocols if needed.
• As an APC-conjugated antibody, it is designed for flow cytometry; optimize titers and compensation in multicolor panels using appropriate controls.
• Cross-reactivity is reported for human and cynomolgus; sequence alignment is recommended when extending to other primate species.
• The antibody targets CD20, which exists as two isoforms (14 kDa and 33 kDa); if used in other applications like WB, validate detection of the expected molecular weight bands in B-cell lines.

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